Structure of PDB 4qz8 Chain A

Receptor sequence

>4qz8A (length=346) Species: 10090 (Mus musculus)

KKISQYACQRRTTLNNYNQLFTDALDILAENDELRENEGSCLAFMRASSV
LKSLPFPITSMKDTEGIPCLGDKVKSIIEGIIEDGESSEAKAVLNDERYK
SFKLFTSVFGVGLKTAEKWFRMGFRTLSKIQSDKSLRFTQMQKAGFLYYE
DLVSCVNRPEAEAVSMLVKEAVVTFLPDALVTMTGGFRRGKMTGHDVDFL
ITSPEATEDEEQQLLHKVTDFWKQQGLLLYCDILESTFVALDHFQKCFLI
LKLDHGRVHSGKGWKAIRVDLVMCPYDRRAFALLGWTGSRQFERDLRRYA
THERKMMLDNHALYDRTKRVFLEAESEEEIFAHLGLDYIEPWERNA

3D structure

• PDB: 4qz8 Structural basis for a novel mechanism of DNA bridging and alignment in eukaryotic DSB DNA repair.
• Chain: A
• Resolution: 2.7 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D343 D345 D434
• Catalytic site (residue number reindexed from 1): D196 D198 D270
• Enzyme Commision number: 2.7.7.31: DNA nucleotidylexotransferase.
  3.1.11.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	G186 S187 A190 C216 G218 K220 V221	G39 S40 A43 C69 G71 K73 V74
BS02	dna	A	L189 V395 A397 R454 R458 R461 H466	L42 V239 A240 R290 R294 R297 H302
BS03	dna	A	F256 G257 G259 K261 T262 M288 L398 F405 R432 W450	F109 G110 G112 K114 T115 M141 L241 F248 R268 W286
BS04	MG	A	D343 D345	D196 D198
BS05	DCT	A	G333 R336 K338 G341 H342 D343 D345 G449 W450 G452 R454 E457	G186 R189 K191 G194 H195 D196 D198 G285 W286 G288 R290 E293

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:4qz8, PDBe:4qz8, PDBj:4qz8
• PDBsum: 4qz8
• PubMed: 25762590
• UniProt: P09838|TDT_MOUSE DNA nucleotidylexotransferase (Gene Name=Dntt)