Structure of PDB 4qei Chain A

Receptor sequence

>4qeiA (length=562) Species: 9606 (Homo sapiens)

IVDRAKMGDTLKRRFFYDQAFAIYGGVSGLYDFGPVGCALKNNIIQTWRQ
HFIQEEQILEIDCTMLTPEPVLKTSGHVDKFADFMVKDVKNGERFRADHL
LKAHLQKLQQELADLFVNFNLMFKTFIGPGGNMPGYLRPETAQGIFLNFK
RLLEFNQGKLPFAAAQIGNSFRNEISPRSGLIRVREFTMAEIEHFVDPSE
KDHPKFQNVADLHLYLYSAKAQVSGQSARKMRLGDAVEQGVINNTVLGYF
IGRIYLYLTKVGISPDKLRFRQHMEDCWDAESKTSYGWIEIVGCADRSCY
DLSCHARATKVPLVAEKPLKEPKTVNVVQFEPSKGAIGKAYKKDAKLVME
YLAICDECYITEMEMLLNEKGEFTIETQLTKDMINVKRFQKTLYVEEVVP
NVIEPSFGLGRIMYTVFEHTFHVREGDEQRTFFSFPAVVAPFKCSVLPLS
QNQEFMPFVKELSEALTRHGVSHKVDDSSGSIGRRYARTDEIGVAFGVTI
DFDTVNKTPHTATLRDRDSMRQIRAEISELPSIVQDLANGNITWADVEAR
YPLFEGQETGKK

3D structure

• PDB: 4qei Large Conformational Changes of Insertion 3 in Human Glycyl-tRNA Synthetase (hGlyRS) during Catalysis
• Chain: A
• Resolution: 2.875 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.-
  6.1.1.14: glycine--tRNA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	D72 R76 Q82 G88 G89 S91 I280 D545 Q547 R548 L567 S568 N570 S596 S597 S599 G601 R602 Y604 A605 R606 E609 T617 D619 F620 T631 R633 M638 Q640 F672 Q675	D9 R13 Q19 G25 G26 S28 I175 D427 Q429 R430 L449 S450 N452 S478 S479 S481 G483 R484 Y486 A487 R488 E491 T499 D501 F502 T513 R515 M520 Q522 F554 Q557	PDBbind-CN: Kd=15.84uM
BS02	AMP	A	R277 I287 V289 F292 M294 E403 I404 G526 R529	R172 I182 V184 F187 M189 E290 I291 G408 R411

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004812 aminoacyl-tRNA ligase activity
• GO:0004820 glycine-tRNA ligase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006418 tRNA aminoacylation for protein translation
• GO:0006426 glycyl-tRNA aminoacylation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:4qei, PDBe:4qei, PDBj:4qei
• PDBsum: 4qei
• PubMed: 26797133
• UniProt: P41250|GARS_HUMAN Glycine--tRNA ligase (Gene Name=GARS1)