Structure of PDB 4pxq Chain A

Receptor sequence

>4pxqA (length=516) Species: 7955 (Danio rerio)

GVRYEEIDCLINDDATIKGRREGSEVYMPFSWMEKYFEVYGKVVQYDGYD
RFEFSHSYSKVYAQREQYHPNGVFMSFEGYNVEVRDRVKCISGVEGVPLS
TQWGPQGYFYAIQIAQYGLSHYSKNLTERPPHVEVYDTAEERDSRSSAWT
VPKGCSLTRVYDKTRATSVREFSAPENSEGVSLPLGNTKDFIISFDLKFT
SNGSVSVILETTEKGPPFVIHYVTTTQLILLKDRDITYGIGPRTTWTTVT
RDLLTDLRKGIGLSNTKAVKATKTMPRRVVKLVVHGTGTIDNITISTTSH
MAAFYAASDWLVRNQDERGGWPIMVTRKLGEGFRALEPGWYSAMAQGQAM
STLVRAYLMTKDDRYLKAALRATGPFKLPSEQHGVKAVFMNKYDWYEEYP
TIPSSFVLNGFIYSLIGLFDLAQTAGEKLGRDAGQLYSKGMESLKVMLPL
YDTGSGTIYDLRHFILGTAPNLARWDYHTTHINQLQLLGTIDNSPIFRDS
VKRWKSYLKGGRAKHN

3D structure

• PDB: 4pxq Structural and Functional Study of d-Glucuronyl C5-epimerase.
• Chain: A
• Resolution: 2.2 Å

Enzymatic activity

• Enzyme Commision number: 5.1.3.17: heparosan-N-sulfate-glucuronate 5-epimerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SGN	A	R396 K397 G399 E400	R327 K328 G330 E331
BS02	SGN	A	R531 N540	R462 N471
BS03	IDS	A	R543 Y546	R474 Y477
BS04	SGN	A	R154 D155 R156 Q182 R543	R85 D86 R87 Q113 R474
BS05	UAP	A	Y149 R154 Q185 Y482 Y546 T549	Y80 R85 Q116 Y413 Y477 T480

Gene Ontology

Molecular Function

• GO:0016853 isomerase activity
• GO:0042802 identical protein binding
• GO:0046872 metal ion binding
• GO:0047464 heparosan-N-sulfate-glucuronate 5-epimerase activity

Biological Process

• GO:0015012 heparan sulfate proteoglycan biosynthetic process
• GO:0030210 heparin biosynthetic process

Cellular Component

• GO:0000139 Golgi membrane
• GO:0005794 Golgi apparatus
• GO:0016020 membrane

External links

• PDB: RCSB:4pxq, PDBe:4pxq, PDBj:4pxq
• PDBsum: 4pxq
• PubMed: 25568314
• UniProt: F1QR43|GLCEB_DANRE D-glucuronyl C5-epimerase B (Gene Name=glceb)