Structure of PDB 4pxh Chain A

Receptor sequence
>4pxhA (length=417) Species: 1001349 (Streptomyces sp. Acta 2897) [Search protein sequence]
PIPDDISTINLTDPRTYEVNDLSEYWRQLRTTRPLYWHPPVGDAPGFWVV
SRYADVMALYKDNKKLTSEKGNVLVTLLAGGDSAAGKMLAVTDGAMHRGL
RNVLLKSFSPQALKPIVDQIRVNTTRLVVDAARRGECDFAADVAEQIPLN
TISDLLGVPAADREFLLKLNKSALSSEDADQSATDAWLARNEILLYFSEL
VAERRAKPTEDVISVLANSMVDGKPLTEEVIVLNCYSLILGGDETSRLSM
IDSVQTFTQYPDQWELLRDGKVTLESATEEVLRWATPAMHFGRRAVTDME
LHGQVIAAGDVVTLWNNSANRDEEVFADPYAFDLNRSPNKHITFGYGPHF
CLGAYLGRAEVHALLDALRTYTTGFEITGEPQRIHSNFLTGLSRLPVRIQ
PNEAAIAAYDSDNGVRS
3D structure
PDB4pxh The structure of a transient complex of a nonribosomal Peptide synthetase and a cytochrome p450 monooxygenase.
ChainA
Resolution2.7 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) S181 G247 E250 T251 S252 C357 L358 G359 E366 L395
Catalytic site (residue number reindexed from 1) S175 G241 E244 T245 S246 C351 L352 G353 E360 L389
Enzyme Commision number 1.14.14.1: unspecific monooxygenase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 HEM A Y66 L95 A96 R107 F114 G247 G248 T251 P293 F297 R299 T349 F350 G351 H355 C357 L358 G359 Y60 L89 A90 R101 F108 G241 G242 T245 P287 F291 R293 T343 F344 G345 H349 C351 L352 G353
BS02 KH4 A D88 S89 L180 W193 Y242 G247 F297 F394 D82 S83 L174 W187 Y236 G241 F291 F388
Gene Ontology
Molecular Function
GO:0004497 monooxygenase activity
GO:0005506 iron ion binding
GO:0008395 steroid hydroxylase activity
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0020037 heme binding
GO:0036199 cholest-4-en-3-one 26-monooxygenase activity
GO:0046872 metal ion binding
Biological Process
GO:0006707 cholesterol catabolic process

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Molecular Function
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Biological Process
External links
PDB RCSB:4pxh, PDBe:4pxh, PDBj:4pxh
PDBsum4pxh
PubMed25044735
UniProtF2YRY7

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