Structure of PDB 4pul Chain A

Receptor sequence
>4pulA (length=367) Species: 264203 (Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821) [Search protein sequence]
RPRFSFSIAAREGKARTGTIEMKRGVIRTPAFMPVGTAATVKALKPETVR
ATGADIILGNTYHLMLRPGAERIAKLGGLHSFMGWDRPILTNSGGYQVMS
LSSLTKQSEEGVTFKSHMLSPERSIEIQHLLGSDIVMAFDECTPYPATPS
RAASSMERSMRWAKRSRDAFDSRKEQAENAALFGIQQGSVFENLRQQSAD
ALAEIGFDGYAVGGLAVGEGQDEMFRVLDFSVPMLPDDKPHYLMGVGKPD
DIVGAVERGIDMFDCVLPTRSGRNGQAFTWDGPINIRNARFSEDLKPLDS
ECHCAVCQKWSRAYIHHLIRAGEILGAMLMTEHNIAFYQQLMQKIRDSIS
EGRFSQFAQDFRARYFA
3D structure
PDB4pul Chasing Protons: How Isothermal Titration Calorimetry, Mutagenesis, and pKa Calculations Trace the Locus of Charge in Ligand Binding to a tRNA-Binding Enzyme.
ChainA
Resolution1.654 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) N102 D280 C318 C320 C323 H349
Catalytic site (residue number reindexed from 1) N92 D264 C302 C304 C307 H333
Enzyme Commision number 2.4.2.29: tRNA-guanosine(34) preQ1 transglycosylase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ZN A C318 C320 C323 H349 C302 C304 C307 H333
BS02 2WU A Y106 D156 C158 G229 L231 A232 M260 G261 Y96 D140 C142 G213 L215 A216 M244 G245 MOAD: Kd=146.1nM
PDBbind-CN: -logKd/Ki=6.84,Kd=146.1nM
Gene Ontology
Molecular Function
GO:0008479 tRNA-guanosine(34) queuine transglycosylase activity
GO:0016757 glycosyltransferase activity
GO:0016763 pentosyltransferase activity
GO:0046872 metal ion binding
Biological Process
GO:0002099 tRNA wobble guanine modification
GO:0006400 tRNA modification
GO:0008033 tRNA processing
GO:0008616 queuosine biosynthetic process
GO:0101030 tRNA-guanine transglycosylation
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:4pul, PDBe:4pul, PDBj:4pul
PDBsum4pul
PubMed24955548
UniProtP28720|TGT_ZYMMO Queuine tRNA-ribosyltransferase (Gene Name=tgt)

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