Structure of PDB 4pkw Chain A

Receptor sequence
>4pkwA (length=491) Species: 1392 (Bacillus anthracis) [Search protein sequence]
SRYEKWEKIKQHYQHWSDSLSEEGRGLLKKLQIPIEPKKDDIIHSLSQEE
KELLKRIQIDSSDFLSTEEKEFLKKLQIDIRDLSEKEKEFLKKLKLDIQP
YDINQRLQDTGGLIDSPSINLDVRKQYKRDIQNIDALLHQSIGSTLYNKI
YLYENMNINNLTATLGADLVDSTDNTKINRGIFNEFKKNFKYSISSNYMI
VDINERPALDNERLKWRIQLSPDTRAGYLENGKLILQRNIGLEIKDVQII
KQSEKEYIRIDAKVVPKSKIDTKIQEAQLNINQEWNKALGLPKYTKLITF
NVHNRYASNIVESAYLILNEWKNNIQSDLIKKVTNYLVDGNGRFVFTDIT
LPNIAEQYTHQDEIYEQVHSKGLYVPESRSILLHGPSKGVELRNDSEGFI
HEFGHAVDDYAGYLLDKNQSDLVTNSKKFIDIFKEEGSNLTSYGRTNEAE
FFAEAFRLMHSTDHAERLKVQKNAPKTFQFINDQIKFIINS
3D structure
PDB4pkw Anthrax toxin lethal factor domain 3 is highly mobile and responsive to ligand binding.
ChainA
Resolution1.75 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) H686 E687 H690 Y728 E735
Catalytic site (residue number reindexed from 1) H401 E402 H405 Y443 E450
Enzyme Commision number 3.4.24.83: anthrax lethal factor endopeptidase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ZN A H686 H690 E735 H401 H405 E450
BS02 GM6 A D328 F329 V653 H654 S655 K656 G657 H686 E687 H690 Y728 E735 D63 F64 V368 H369 S370 K371 G372 H401 E402 H405 Y443 E450 MOAD: ic50=22.5uM
PDBbind-CN: -logKd/Ki=4.65,IC50=22.5uM
Gene Ontology
Molecular Function
GO:0008237 metallopeptidase activity

View graph for
Molecular Function
External links
PDB RCSB:4pkw, PDBe:4pkw, PDBj:4pkw
PDBsum4pkw
PubMed25372673
UniProtP15917|LEF_BACAN Lethal factor (Gene Name=lef)

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