Structure of PDB 4pj3 Chain A

Receptor sequence

>4pj3A (length=1335) Species: 9606 (Homo sapiens)

NAEFVTQLACKYWAPHIKKKSPFDIKVIEDIYEKEIVKSRFAIRKIMLLE
FSQYLENYLWMNYSPEVSSKAYLMSICCMVNEKFRENVPAWEIFKKKPDH
FPFFFKHILKAALAETDGEFSLHEQTVLLLFLDHCFNSLEVDLIRSQVQQ
LISLPMWMGLQLARLELELKKTPKLRKFWNLIKKNDEKMDPEAREQAYQE
RRFLSQLIQKFISVLKSVPLSEPVTMDKVHYCERFIELMIDLEALLPTRR
WFNTILDDSHLLVHCYLSNLVRREEDGHLFSQLLDMLKFYTGFEINDQTG
NALTENEMTTIHYDRITSLQRAAFAHFPELYDFALSNVAEVDTRESLVKF
FGPLSSNTLHQVASYLCLLPTLPKNEDTTFDKEFLLELLVSRHERRISQI
QQLNQMPLYPTEKIIWDENIVPTEYYSGEGCLALPKLNLQFLTLHDYLLR
NFNLFRLESTYEIRQDIEDSVSRMKPWQSEYGGVVFGGWARMAQPIVAFT
VVEVAKPNIGENWPTRVRADVTINLNVRDHIKDEWEGLRKHDVCFLITVR
PTKPYGTKFDRRRPFIEQVGLVYVRGCEIQGMLDDKGRVIEPRPNLRGES
RTFRVFLDPNQYQQDMTNTIQNGAEDVYETFNIIMRRKPKENNFKAVLET
IRNLMNTDCVVPDWLHDIILGYGDPSSAHYSKMPNQIATLDFNDTFLSIE
HLKASFPGHNVKVTVEDPALQIPPFRITFPVEAKTLIVEPHVIPNRGPYP
YNQPKRNTIQFTHTQIEAIRAGMQPGLTMVVGPPGTGKTDVAVQIISNIY
HNFPEQRTLIVTHSNQALNQLFEKIMALDIDERHLLRLGHGEEELETEKD
FSRYGRVNYVLARRIELLEEVKRLQKSLGVPGDASYTCETAGYFFLYQVM
SRWEEYISKVKNPDVTEVSTFFPFHEYFANAPQPIFKGRSYEEDMEIAEG
CFRHIKKIFTQLEEFRASELLRSGLDRSKYLLVKEAKIIAMTCTHAALKR
HDLVKLGFKYDNILMEEAAQILEIETFIPLLLQNPQDGFSRLKRWIMIGD
HHQLPPVIKNMAFQKYSNMEQSLFTRFVRVGVPTVDLDAQGRARASLCNL
YNWRYKNLGNLPHVQLLPEFSTANAGLLYDFQLINVEDFQGVGESEPNPY
FYQNLGEAEYVVALFMYMCLLGYPADKISILTTYNGQKHLIRDIINRRCG
NNPLIGRPNKVTTVDRFQGQQNDYILLSLVRTRAVGHLRDVRRLVVAMSR
ARLGLYIFARVSLFQNCFELTPAFSQLTARPLHLHIIPTEPFPTTRKNGE
RPSHEVQIIKNMPQMANFVYNMYMHLIQTTHHYHQ

3D structure

• PDB: 4pj3 The RNA helicase Aquarius exhibits structural adaptations mediating its recruitment to spliceosomes.
• Chain: A
• Resolution: 2.3 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ANP	A	I800 Q801 Q806 P825 G826 G828 K829 T830 D831 L1157	I759 Q760 Q765 P784 G785 G787 K788 T789 D790 L1111

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003724 RNA helicase activity
• GO:0003727 single-stranded RNA binding
• GO:0003729 mRNA binding
• GO:0004386 helicase activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016787 hydrolase activity
• GO:0016887 ATP hydrolysis activity
• GO:0034458 3'-5' RNA helicase activity

Biological Process

• GO:0000398 mRNA splicing, via spliceosome
• GO:0006397 mRNA processing
• GO:0008380 RNA splicing

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005681 spliceosomal complex
• GO:0016020 membrane
• GO:0071007 U2-type catalytic step 2 spliceosome
• GO:0071013 catalytic step 2 spliceosome

External links

• PDB: RCSB:4pj3, PDBe:4pj3, PDBj:4pj3
• PDBsum: 4pj3
• PubMed: 25599396
• UniProt: O60306|AQR_HUMAN RNA helicase aquarius (Gene Name=AQR)