Structure of PDB 4op0 Chain A

Receptor sequence

>4op0A (length=259) Species: 83332 (Mycobacterium tuberculosis H37Rv)

MADRDRLRPPLDERSLRDQLIGAGSGWRQLDVVAQTGSTNADLLARAASG
ADIDGVVLIAEHQTAGRGRHGRGWAATARAQIILSVGVRVVDVPVQAWGW
LSLAAGLAVLDSVAPLITGLKWPNDVLARGGKLAGILAEVAQPFVVLGVG
LNVTQAPEEVDPDATSLLDLGVAAPDRNRIASRLLRELEARIIQWRNANP
QLAADYRARSLTIGSRVRVELPGGQDVVGIARDIDDQGRLCLDVGGRTVV
VSAGDVVHL

3D structure

• PDB: 4op0 Active site conformational changes upon reaction intermediate biotinyl-5'-AMP binding in biotin protein ligase from Mycobacterium tuberculosis.
• Chain: A
• Resolution: 1.7 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): R69 K138
• Catalytic site (residue number reindexed from 1): R69 K132
• Enzyme Commision number: 6.3.4.15: biotin--[biotin carboxyl-carrier protein] ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BT5	A	S38 T39 N40 Q63 G66 R67 R69 R72 G73 W74 A75 I83 K138 G141 I142 V155 G156 N158 V166 D167 A170	S38 T39 N40 Q63 G66 R67 R69 R72 G73 W74 A75 I83 K132 G135 I136 V149 G150 N152 V160 D161 A164

Gene Ontology

Molecular Function

• GO:0004077 biotin--[biotin carboxyl-carrier protein] ligase activity
• GO:0005524 ATP binding
• GO:0009374 biotin binding
• GO:0016874 ligase activity
• GO:0042803 protein homodimerization activity

Biological Process

• GO:0008284 positive regulation of cell population proliferation
• GO:0036211 protein modification process

Cellular Component

• GO:0005737 cytoplasm
• GO:0032991 protein-containing complex

External links

• PDB: RCSB:4op0, PDBe:4op0, PDBj:4op0
• PDBsum: 4op0
• PubMed: 24723382
• UniProt: I6YFP0|BIRA_MYCTU Biotin--[acetyl-CoA-carboxylase] ligase (Gene Name=birA)