Structure of PDB 4oli Chain A

Receptor sequence

>4oliA (length=539) Species: 9606 (Homo sapiens)

LSFHRVDQKEITQLSHLGQGTRTNVYEGRLRVELRVVLKVLDPSHHDIAL
AFYETASLMSQVSHTHLAFVHGVCVRGPENIMVTEYVEHGPLDVWLRRER
GHVPMAWKMVVAQQLASALSYLENKNLVHGNVCGRNILLARLGLAEGTSP
FIKLSDPGVGLGALSREERVERIPWLAPECLPGLSTAMDKWGFGATLLEI
CFDGEAPLQSRSPSEKEHFYQRQHRLPEPSCPQLATLTSQCLTYEPTQRP
SFRTILRDLTRLQPPTVFHKRYLKKIRDLGEGHFGKVSLYCYDPTNDGTG
EMVAVKALHRSGWKQEIDILRTLYHEHIIKYKGCCEDQGEKSLQLVMEYV
PLGSLRDYLPRHSIGLAQLLLFAQQICEGMAYLHSQHYIHRNLAARNVLL
DNDVKIGDFGLAKAVPEGHEYYRVREDGDSPVFWYAPECLKEYKFYYASD
VWSFGVTLYELLTHCDSSQSPPTKFLELIGMTVLRLTELLERGERLPRPD
KCPCEVYHLMKNCWETEASFRPTFENLIPILKTVHEKYQ

3D structure

• PDB: 4oli Structure of the pseudokinase-kinase domains from protein kinase TYK2 reveals a mechanism for Janus kinase (JAK) autoinhibition.
• Chain: A
• Resolution: 2.8 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): N734 C736 R738 N739 T751 D759 N1023 A1025 N1028 D1041
• Catalytic site (residue number reindexed from 1): N131 C133 R135 N136 T148 D156 N392 A394 N397 D408
• Enzyme Commision number: 2.7.10.2: non-specific protein-tyrosine kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	2TT	A	G596 Q597 V603 V640 Y689 V690 E691 G693 L741	G18 Q19 V25 V37 Y86 V87 E88 G90 L138
BS02	2TT	A	L903 E905 Y980 V981 G984 L1030 G1040 D1041	L279 E281 Y349 V350 G353 L399 G407 D408

Gene Ontology

Molecular Function

• GO:0004672 protein kinase activity
• GO:0004715 non-membrane spanning protein tyrosine kinase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006468 protein phosphorylation
• GO:0035556 intracellular signal transduction

Cellular Component

• GO:0016020 membrane

External links

• PDB: RCSB:4oli, PDBe:4oli, PDBj:4oli
• PDBsum: 4oli
• PubMed: 24843152
• UniProt: P29597|TYK2_HUMAN Non-receptor tyrosine-protein kinase TYK2 (Gene Name=TYK2)