Structure of PDB 4o5u Chain A

Receptor sequence
>4o5uA (length=521) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIAELSDKV
TFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGH
PSKEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIK
HTAIDGGTFQNEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAE
KRAAEELNKRDAYDVLIVGSGPAGAAAAIYSARKGIRTGLMGERFGGQIL
DTVDIENYISVPKTEGQKLAGALKVHVDEYDVDVIDSQSASKLIPAAVEG
GLHQIETASGAVLKARSIIVATGAKWRNMNVPGEDQYRTKGVTYCPHCDG
PLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKADQVLQDKL
RSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIA
TGEGAKASLSAFDYLIRTKTA
3D structure
PDB4o5u Structure, mechanism and ensemble formation of the alkylhydroperoxide reductase subunits AhpC and AhpF from Escherichia coli
ChainA
Resolution2.65 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) C345 C348 D349
Catalytic site (residue number reindexed from 1) C345 C348 D349
Enzyme Commision number 1.8.1.-
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 FAD A G221 P222 A223 E243 G247 Q248 T252 N257 S289 A290 T322 G323 W326 C348 G487 D488 Q496 I497 G221 P222 A223 E243 G247 Q248 T252 N257 S289 A290 T322 G323 W326 C348 G487 D488 Q496 I497
Gene Ontology
Molecular Function
GO:0004791 thioredoxin-disulfide reductase (NADPH) activity
GO:0008785 alkyl hydroperoxide reductase activity
GO:0016491 oxidoreductase activity
GO:0016668 oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor
GO:0050660 flavin adenine dinucleotide binding
GO:0051287 NAD binding
GO:0071949 FAD binding
Biological Process
GO:0000302 response to reactive oxygen species
GO:0006979 response to oxidative stress
GO:0042744 hydrogen peroxide catabolic process
GO:0045454 cell redox homeostasis
GO:0098869 cellular oxidant detoxification
Cellular Component
GO:0005829 cytosol
GO:0009321 alkyl hydroperoxide reductase complex

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Molecular Function
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Cellular Component
External links
PDB RCSB:4o5u, PDBe:4o5u, PDBj:4o5u
PDBsum4o5u
PubMed25372677
UniProtP35340|AHPF_ECOLI Alkyl hydroperoxide reductase subunit F (Gene Name=ahpF)

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