Structure of PDB 4nm5 Chain A

Receptor sequence

>4nm5A (length=361) Species: 9606 (Homo sapiens)

GSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGNGSFGVVYQA
KLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKK
DEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQLFRSLAYIH
SFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYR
APELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVL
GTPTREQIREMNPNYTEFKFPQIKAHPWTKVFRPRTPPEAIALCSRLLEY
TPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTTQELSSNPPL
ATILIPPHARH

3D structure

• PDB: 4nm5 Structural basis of GSK-3 inhibition by N-terminal phosphorylation and by the Wnt receptor LRP6.
• Chain: A
• Resolution: 2.3 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D181 K183 N186 D200 S219
• Catalytic site (residue number reindexed from 1): D158 K160 N163 D177 S196
• Enzyme Commision number: 2.7.11.1: non-specific serine/threonine protein kinase.
  2.7.11.26: [tau protein] kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	V263 D264 Y288 F291 F293 P294 I296	V240 D241 Y265 F268 F270 P271 I273
BS02	peptide	A	R92 F93 K94 R96 R180 K205 N213 V214 Y216	R69 F70 K71 R73 R157 K182 N190 V191 Y193
BS03	ADP	A	F67 A83 K85 D133 Y134 V135 L188 D200	F44 A60 K62 D110 Y111 V112 L165 D177
BS04	MG	A	N186 D200	N163 D177

Gene Ontology

Molecular Function

• GO:0004672 protein kinase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006468 protein phosphorylation

External links

• PDB: RCSB:4nm5, PDBe:4nm5, PDBj:4nm5
• PDBsum: 4nm5
• PubMed: 24642411
• UniProt: P49841|GSK3B_HUMAN Glycogen synthase kinase-3 beta (Gene Name=GSK3B)