Structure of PDB 4n99 Chain A

Receptor sequence
>4n99A (length=356) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
MKFTVEREHLLKPLQQVSGPLPILGNLLLQVADGTLSLTGTDLEMEMVAR
VALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSGRSRF
SLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLN
GMLFETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRM
LNPLRVQIGSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGC
DLLKQAFARAAILSNEKFRGVRLYVSENQLKITANNPEQEEAEEILDVTY
SGAEMEIGFNVSYVLDVLNALKCENVRMMLTDSVSSVQIEDAASQSAAYV
VMPMRL
3D structure
PDB4n99 Discovery of lead compounds targeting the bacterial sliding clamp using a fragment-based approach.
ChainA
Resolution2.3 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.7.7: DNA-directed DNA polymerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 2J1 A H175 P242 V247 S346 H169 P232 V237 S336 MOAD: Ki=216uM
PDBbind-CN: -logKd/Ki=3.67,Ki=216uM
BS02 CA A V57 A58 V51 A52
BS03 CA A R282 L366 R272 L356
BS04 CA A G228 D229 G218 D219
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003887 DNA-directed DNA polymerase activity
GO:0005515 protein binding
GO:0008408 3'-5' exonuclease activity
GO:0042802 identical protein binding
GO:0042803 protein homodimerization activity
Biological Process
GO:0006260 DNA replication
GO:0006261 DNA-templated DNA replication
GO:0006271 DNA strand elongation involved in DNA replication
GO:0006974 DNA damage response
GO:0030174 regulation of DNA-templated DNA replication initiation
GO:0032297 negative regulation of DNA-templated DNA replication initiation
GO:0042276 error-prone translesion synthesis
GO:0044787 bacterial-type DNA replication
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0009360 DNA polymerase III complex
GO:0030894 replisome
GO:1990078 replication inhibiting complex
GO:1990085 Hda-beta clamp complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:4n99, PDBe:4n99, PDBj:4n99
PDBsum4n99
PubMed24592885
UniProtP0A988|DPO3B_ECOLI Beta sliding clamp (Gene Name=dnaN)

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