Structure of PDB 4n56 Chain A

Receptor sequence

>4n56A (length=494) Species: 271 (Thermus aquaticus)

LEEAPWPPPEGAFVGFVLSRKEPMWADLLALAAARGGRVHRAPEPYKALR
DLKEARGLLAKDLSVLALREGLGLPPGDDPMLLAYLLDPSNTTPEGVARR
YGGEWTEEAGERAALSERLFANLWGRLEGEERLLWLYREVERPLSAVLAH
MEATGVRLDVAYLRALSLEVAEEIARLEAEVFRLAGHPFNLNSRDQLERV
LFDELGLPAIGKTEKTGKRSTSAAVLEALREAHPIVEKILQYRELTKLKS
TYIDPLPDLIHPRTGRLHTRFNQTATATGRLSSSDPNLQNIPVRTPLGQR
IRRAFIAEEGWLLVALDYSQIELRVLAHLSGDENLIRVFQEGRDIHTETA
AKTINFGVLYGFPKVRAWLEKTLEEGRRRGYVETLFGRRRYVPDLEARVK
SVREAAERMAFNMPVQGTAADLMKLAMVKLFPRLEEMGARMLLQVHDELV
LEAPKERAEAVARLAKEVMEGVYPLAVPLEVEVGIGEDWLSAKE

3D structure

• PDB: 4n56 A Conservative Isoleucine to Leucine Mutation Causes Major Rearrangements and Cold Sensitivity in KlenTaq1 DNA Polymerase.
• Chain: A
• Resolution: 2.2 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	R487 T506 K508 T509 S513 T514 S515 A516 R536 K540 Y545 R573 N583 I584 P585 V586 R587 H784	R194 T213 K215 T216 S220 T221 S222 A223 R243 K247 Y252 R280 N290 I291 P292 V293 R294 H446
BS02	dna	A	N483 N485 S486 Q489 S543 T544 T569 A570 T571 R573 S575 S576 S577 D578 N580 F667 R728 R746 M747 N750 Q754	N190 N192 S193 Q196 S250 T251 T276 A277 T278 R280 S282 S283 S284 D285 N287 F356 R390 R408 M409 N412 Q416
BS03	MG	A	D610 E786	D317 E448

Gene Ontology

Molecular Function

• GO:0001882 nucleoside binding
• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication
• GO:0006281 DNA repair

External links

• PDB: RCSB:4n56, PDBe:4n56, PDBj:4n56
• PDBsum: 4n56
• PubMed: 25537790
• UniProt: P19821|DPO1_THEAQ DNA polymerase I, thermostable (Gene Name=polA)