Structure of PDB 4n48 Chain A

Receptor sequence

>4n48A (length=401) Species: 9606 (Homo sapiens)

VDWRDEPEPSACEQVSWFPECTTEIPDTQEMSDWMVVGKRKMIIEDETEF
CGEELLHSVLQCKSVFDVLDGEEMRRARTRANPYEMIRGVFFLNRAAMKM
ANMDFVFDRMFTNPRDSYGKPLVKDRELLYFADVCAGPGGFSEYVLWRKK
WHAKGFGMTLKGPNDFKLEDFSELFEPYYGEGGIDGDGDITRPENISAFR
NFVLDNTDRKGVHFLMADGGFSVEGQENLQEILSKQLLLCQFLMALSIVR
TGGHFICKTFDLFTPFSVGLVYLLYCCFERVCLFKPITSRPANSERYVVC
KGLKVGIDDVRDYLFAVNIKLNQLRNTDSDVNLVVPLEVIKGDHEFTDYM
IRSNESHCSLQIKALAKIHAFVQDTTLSEPRQAEIRKECLRLWGIPDQAR
V

3D structure

• PDB: 4n48 Structural analysis of human 2'-O-ribose methyltransferases involved in mRNA cap structure formation.
• Chain: A
• Resolution: 2.704 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.57: methyltransferase cap1.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	N234 R235 K239 F367 S368 K404	N94 R95 K99 F221 S222 K258
BS02	SAM	A	A236 C277 A278 G279 G281 G282 F283 L302 N306 G334 D335 I336 D364 G366	A96 C135 A136 G137 G139 G140 F141 L160 N164 G188 D189 I190 D218 G220
BS03	MGT	A	K203 S204 F206 D207 M214 R218 E373 N439	K63 S64 F66 D67 M74 R78 E227 N293

Gene Ontology

Molecular Function

• GO:0008168 methyltransferase activity

Biological Process

• GO:0032259 methylation

External links

• PDB: RCSB:4n48, PDBe:4n48, PDBj:4n48
• PDBsum: 4n48
• PubMed: 24402442
• UniProt: Q8N1G2|CMTR1_HUMAN Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 (Gene Name=CMTR1)