Structure of PDB 4n47 Chain A

Receptor sequence

>4n47A (length=666) Species: 274 (Thermus thermophilus) [Search protein sequence]

GKTEVFLNRFALRPLNPEELRPWRLEVVLDPPPGREEVYPLLAQVARRAG
GVTVRMGDGLASWSPPEVLVLEGTLARMGQTYAYRLYPKGRRPLDPKDPG
ERSVLSALARRLLQERLRRLEGVWVEGLAVYRREHARGPGWRVLGGAVLD
LWVSDSGAFLLEVDPAYRILCEMSLEAWLAQGHPLPKRVRNAYDRRTWEL
LRLGEEDPKELPLPGGLSLLDYHASKGRLQGREGGRVAWVADPKDPRKPI
PHLTGLLVPVLTLEDLSLPWEERRRRTREIASWIGRRLGLGTPEAVRAQA
YRLSIPKLMGRRAVSKPADALRVGFYRAQETALALLRLDGAQGWPEFLRR
ALLRAFGASGASLRLHTLHAHPSQGLAFREALRKAKEEGVQAVLVLTPPM
AWEDRNRLKALLLREGLPSQILNVPLREEERHRWENALLGLLAKAGLQVV
ALSGAYPAELAVGFDAGGRESFRFGGAACAVGGHLLWTLPEAERIPQEVV
WDLLEETLWAFRRKAGRLPSRVLLLRDGRVPQDEFALALEALAREGIAYD
LVSVRKSGGGRVYPVQGRLADGLYVPLEDKTFLLLTVHRDFRGTPRPLKL
VHEAGDTPLEALAHQIFHLTRLYPASGFAFPRLPAPLHLADRLVKEVGRL
GIRHLKEVDREKLFFV

3D structure

PDB

4n47 Structure-based cleavage mechanism of Thermus thermophilus Argonaute DNA guide strand-mediated DNA target cleavage.

Chain

Resolution

2.823 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

3.1.24.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	E268 S328 K329 H445 R574 K575 S576 D590 V606 H607 R608 F610 F647 A648 F649	E264 S315 K316 H432 R555 K556 S557 D571 V587 H588 R589 F591 F628 A629 F630
BS02	dna	A	A170 Y171 R172 I173 Y197 T201 L217 Y226 H227 R232 I254 P255 L265 R286 P412 M413 W415 K422 Q433 I434 N436 R446 N449 K457 R580 R611 T613 P614 Y642 S645 A648 P650 R651 H657 V685	A166 Y167 R168 I169 Y193 T197 L213 Y222 H223 R228 I250 P251 L261 R273 P399 M400 W402 K409 Q420 I421 N423 R433 N436 K444 R561 R592 T594 P595 Y623 S626 A629 P631 R632 H638 V666

Gene Ontology

	Molecular Function
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003723	RNA binding
GO:0004519	endonuclease activity
GO:0004520	DNA endonuclease activity
GO:0030145	manganese ion binding
GO:0046872	metal ion binding

	Biological Process
GO:0006260	DNA replication
GO:0044355	clearance of foreign intracellular DNA

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:4n47, PDBe:4n47, PDBj:4n47
PDBsum	4n47
PubMed	24374628
UniProt	Q746M7\|AGO_THET2 Protein argonaute (Gene Name=ago)

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