Structure of PDB 4n3c Chain A

Receptor sequence

>4n3cA (length=697) Species: 9606 (Homo sapiens)

CPTHADSLNNLANIKREQGNIEEAVRLYRKALEVFPEFAAAHSNLASVLQ
QQGKLQEALMHYKEAIRISPTFADAYSNMGNTLKEMQDVQGALQCYTRAI
QINPAFADAHSNLASIHKDSGNIPEAIASYRTALKLKPDFPDAYCNLAHC
LQIVCDWTDYDERMKKLVSIVADQLEKNRLPSVHPHHSMLYPLSHGFRKA
IAERHGNLCLDKINVLHKPPYEHPKDLKLSDGRLRVGYVSSDFGNHPTSH
LMQSIPGMHNPDKFEVFCYALSPDDGTNFRVKVMAEANHFIDLSQIPCNG
KAADRIHQDGIHILVNMNGYTKGARNELFALRPAPIQAMWLGYPGTSGAL
FMDYIITDQETSPAEVAEQYSEKLAYMPHTFFIGDHANMFPHLKKKAVID
FKIYDNRIVLNGIDLKAFLDSLPDVKIVKMLNMPVIPMNTIAEAVIEMIN
RGQIQITINGFSISNGLATTQINNKAATGEEVPRTIIVTTRSQYGLPEDA
IVYCNFNQLYKIDPSTLQMWANILKRVPNSVLWLLRFPAVGEPNIQQYAQ
NMGLPQNRIIFSPVAPKEEHVRRGQLADVCLDTPLCNGHTTGMDVLWAGT
PMVTMPGETLASRVAASQLTCLGCLELIAKNRQEYEDIAVKLGTDLEYLK
KVRGKVWKQRISSPLFNTKQYTMELERLYLQMWEHYAAGNKPDHMIK

3D structure

• PDB: 4n3c HCF-1 is cleaved in the active site of O-GlcNAc transferase.
• Chain: A
• Resolution: 2.55 Å

Enzymatic activity

• Enzyme Commision number: 2.4.1.255: protein O-GlcNAc transferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	D318 N322 N325 F350 N356 F384 D386 N390 N393 K396 F418 D420 N424 K430 D431 D454 N458 H498 N557 H558 P559 T633 K634 G654 P656 Q839 V895	D6 N10 N13 F38 N44 F72 D74 N78 N81 K84 F106 D108 N112 K118 D119 D142 N146 H186 N245 H246 P247 T321 K322 G342 P344 Q508 V564
BS02	UD1	A	P559 T560 L653 P656 Q839 K842 L866 V895 A896 K898 H901 H920 T921 T922 D925	P247 T248 L341 P344 Q508 K511 L535 V564 A565 K567 H570 H589 T590 T591 D594

External links

• PDB: RCSB:4n3c, PDBe:4n3c, PDBj:4n3c
• PDBsum: 4n3c
• PubMed: 24311690
• UniProt: O15294|OGT1_HUMAN UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit (Gene Name=OGT)