Structure of PDB 4mne Chain A

Receptor sequence
>4mneA (length=286) Species: 9606 (Homo sapiens) [Search protein sequence]
LKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQII
RELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIP
EQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVS
GQLIDSMANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYP
IPPPDAKELELMPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLI
KNPAERADLKQLMVHAFIKRSDAEEVDFAGWLCSTI
3D structure
PDB4mne Structure of the BRAF-MEK Complex Reveals a Kinase Activity Independent Role for BRAF in MAPK Signaling.
ChainA
Resolution2.8483 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D190 K192 S194 N195 D208 D217 T226
Catalytic site (residue number reindexed from 1) D128 K130 S132 N133 D146 D155 T164
Enzyme Commision number 2.7.12.2: mitogen-activated protein kinase kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ACP A G75 A76 G77 N78 V82 A95 K97 M143 M146 S150 Q153 K192 S194 L197 G13 A14 G15 N16 V20 A33 K35 M81 M84 S88 Q91 K130 S132 L135
BS02 MG A N195 D208 N133 D146
BS03 573 A G80 K97 L115 V127 I141 M143 C207 D208 F209 G210 V211 S212 L215 G18 K35 L53 V65 I79 M81 C145 D146 F147 G148 V149 S150 L153 BindingDB: EC50=7.2nM,IC50=35nM
Gene Ontology
Molecular Function
GO:0004672 protein kinase activity
GO:0005524 ATP binding
Biological Process
GO:0006468 protein phosphorylation

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Molecular Function
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Biological Process
External links
PDB RCSB:4mne, PDBe:4mne, PDBj:4mne
PDBsum4mne
PubMed25155755
UniProtQ02750|MP2K1_HUMAN Dual specificity mitogen-activated protein kinase kinase 1 (Gene Name=MAP2K1)

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