Structure of PDB 4mig Chain A

Receptor sequence

>4migA (length=570)

PYDVFIAGSGPIGATFAKLCVDANLRVCMVEIGAADSFTSKPMKQFGPGQ
VPIPGYHKKNEIEYQKDIDRFVNVIKGALSTCSIPTSNNHIATLDPSVVS
NSLDKPFISLGKNPAQNPFVNLGAEAVTRGVGGMSTHWTCATPEFFAPAD
FNAPHRERPKLSTDAAEDARIWKDLYAQAKEIIGTSTTEFDHSIRHNLVL
RKYNDIFQKENVIREFSPLPLACHRLTDPDYVEWHATDRILEELFTDPVK
RGRFTLLTNHRCTKLVFKHYRPGEENEVDYALVEDLLPSVKKIYARSYVV
ACGAVATAQVLANSHIPPERDATIPTPLMPMLGKYITEQPMTFCQVVLDS
SLMEVVRNPPWPGLDWWKEKVARHVEAFPNDPIPIPFRDPEPQVTIKFTE
EHPWHVQIHRDAFSYGAVAENMDTRVIVDYRFFGYTEPQEANELVFQQHY
RDAYDMPQPTFKFTMSQDDRARARRMMDDMCNIALKIGGYLPGSEPQFMT
PGLALHLAGTTRCGLDTQKTVGNTHCKVHNFNNLYVGGNGVIETGFAANP
TLTSICYAIRASNDIIAKFG

3D structure

• PDB: 4mig Crystal structures of Phanerochaete chrysosporium pyranose 2-oxidase suggest that the N-terminus acts as a propeptide that assists in homotetramer assembly.
• Chain: A
• Resolution: 1.8 Å

Enzymatic activity

• Enzyme Commision number: 1.1.3.10: pyranose oxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FDA	A	G22 P23 I24 E43 I44 T149 R150 G151 G154 M155 H158 W159 T160 A162 C283 C332 A336 L552 N596 T598	G10 P11 I12 E31 I32 T128 R129 G130 G133 M134 H137 W138 T139 A141 C262 C302 A306 L505 N549 T551
BS02	G3F	A	T160 Q454 D458 Y462 A551 H553 N596	T139 Q407 D411 Y415 A504 H506 N549

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity
• GO:0016614 oxidoreductase activity, acting on CH-OH group of donors
• GO:0050233 pyranose oxidase activity
• GO:0050660 flavin adenine dinucleotide binding

Cellular Component

• GO:0042597 periplasmic space

External links

• PDB: RCSB:4mig, PDBe:4mig, PDBj:4mig
• PDBsum: 4mig
• PubMed: 24282677
• UniProt: Q6QWR1|P2OX_PHACH Pyranose 2-oxidase (Gene Name=p2ox)