Structure of PDB 4m9d Chain A

Receptor sequence

>4m9dA (length=423) Species: 261594 (Bacillus anthracis str. 'Ames Ancestor')

MSSVVVVGTQWGDEGKGKITDFLSEHAEVVARYQGGNNAGHTIVFGGVKY
KLHLIPSGIFYKEKICVIGNGLVVDPKALLEELKYLHDRGVSTDNLRVSN
RAHVILPYHLKQDELEEASKGDNKIGTTKKGIGPAYMDKAARIGIRMADL
LDREAFKEKLEQNLAQKNRLFEKMYDTEGFSVDEIFEEYFEYGQQIAQYV
CDTSVVLNDALDNNHRVLFEGAQGVMLDIDHGTYPFVTSSNPIAGGVTVG
TGVGPAKVTRVVGVCKAYTSRVGDGPFPTELHDEIGHQIREVGREYGTTT
GRPRRVGWFDSVVVRHARRVSGLTDLSLNSIDVLTGIPTLKICVAYKCDG
KVIDEVPANLNILAKCEPVCEELPGWTEDITGVRSLDELPENARKYVERV
SELTGIQLSMFSVGPDRNQTNIV

3D structure

• PDB: 4m9d The Crystal structure of an adenylosuccinate synthetase from Bacillus anthracis str. Ames Ancestor in complex with AMP.
• Chain: A
• Resolution: 1.821 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D13 K16 G40 H41 Q223
• Catalytic site (residue number reindexed from 1): D13 K16 G40 H41 Q223
• Enzyme Commision number: 6.3.4.4: adenylosuccinate synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	AMP	A	N38 G126 T127 T128 Q223 L227 V237 T238 V272 G273	N38 G126 T127 T128 Q223 L227 V237 T238 V272 G273

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0000287 magnesium ion binding
• GO:0004019 adenylosuccinate synthase activity
• GO:0005525 GTP binding
• GO:0016874 ligase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006164 purine nucleotide biosynthetic process
• GO:0044208 'de novo' AMP biosynthetic process
• GO:0046040 IMP metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:4m9d, PDBe:4m9d, PDBj:4m9d
• PDBsum: 4m9d
• UniProt: Q81JI9|PURA_BACAN Adenylosuccinate synthetase (Gene Name=purA)