Structure of PDB 4m65 Chain A

Receptor sequence

>4m65A (length=316) Species: 274 (Thermus thermophilus)

ITGTSTVGVGRGVLGDQKNINTTYSTYYYLQDNTRGDGIFTYDAKYRTTL
PGSLWADADNQFFASYDAPAVDAHYYAGVTYDYYKNVHNRLSYDGNNAAI
RSSVHYSQGYNNAFWNGSEMVYGDGDGQTFIPLSGGIDVVAHELTHAVTD
YTAGLIYQNESGAINEAISDIFGTLVEFYANKNPDWEIGEDVYTPGISGD
SLRSMSDPAKYGDPDHYSKRYTGTQDNGGVHINSGIINKAAYLISQGGTH
YGVSVVGIGRDKLGKIFYRALTQYLTPTSNFSQLRAAAVQSATDLYGSTS
QEVASVKQAFDAVGVK

3D structure

• PDB: 4m65 Hitting the target: fragment screening with acoustic in situ co-crystallization of proteins plus fragment libraries on pin-mounted data-collection micromeshes
• Chain: A
• Resolution: 1.6 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H142 E143 H146 Y157 E166 D226 H231
• Catalytic site (residue number reindexed from 1): H142 E143 H146 Y157 E166 D226 H231
• Enzyme Commision number: 3.4.24.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	A	D138 E177 D185 E187 E190	D138 E177 D185 E187 E190
BS02	CA	A	E177 N183 D185 E190	E177 N183 D185 E190
BS03	CA	A	D57 D59 Q61	D57 D59 Q61
BS04	CA	A	Y193 T194 I197 D200	Y193 T194 I197 D200
BS05	ZN	A	H142 H146 E166	H142 H146 E166
BS06	ASN	A	F114 W115 H142 E143 H146 Y157 E166 H231	F114 W115 H142 E143 H146 Y157 E166 H231

Gene Ontology

Molecular Function

• GO:0004222 metalloendopeptidase activity
• GO:0008237 metallopeptidase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006508 proteolysis

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:4m65, PDBe:4m65, PDBj:4m65
• PDBsum: 4m65
• UniProt: V5IRV7