Structure of PDB 4lxj Chain A

Receptor sequence

>4lxjA (length=530) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence]

SIVGEALEYVNIGLSHFLALPLAQRISLIIIIPFIYNIVWQLLYSLRKDR
PPLVFYWIPWVGSAVVYGMKPYEFFEECQKKYGDIFSFVLLGRVMTVYLG
PKGHEFVFNAKLADVSAEAAYAHLTTPVFGKGVIYDCPNSRLMEQKKFVK
GALTKEAFKSYVPLIAEEVYKYFRDSKNFRLNERTTGTIDVMVTQPEMTI
FTASRSLLGKEMRAKLDTDFAYLYSDLDKGFTPINFVFPNLPLEHYRKRD
HAQKAISGTYMSLIKERRKNNDIQDRDLIDSLMKNSTYKDGVKMTDQEIA
NLLIGVLMGGQHTSAATSAWILLHLAERPDVQQELYEEQMRVLDGGKKEL
TYDLLQEMPLLNQTIKETLRMHHPLHSLFRKVMKDMHVPNTSYVIPAGYH
VLVSPGYTHLRDEYFPNAHQFNIHRWNNDSASSYSVGEEVDYGFGAISKG
VSSPYLPFGGGRHRCIGEHFAYCQLGVLMSIFIRTLKWHYPEGKTVPPPD
FTSMVTLPTGPAKIIWEKRNPEQKIGGRHH

3D structure

PDB

4lxj Architecture of a single membrane spanning cytochrome P450 suggests constraints that orient the catalytic domain relative to a bilayer.

Chain

Resolution

1.9 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	T318 F463 C470
Catalytic site (residue number reindexed from 1)	T313 F458 C465
Enzyme Commision number	1.14.14.154: sterol 14alpha-demethylase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEM	A	Y126 Y140 K151 V311 G314 G315 T318 L374 P379 L383 R385 P462 F463 H468 C470 G472	Y121 Y135 K146 V306 G309 G310 T313 L369 P374 L378 R380 P457 F458 H463 C465 G467
BS02	LAN	A	Y126 F134 Y140 F236 F241 G314 L380 H381 F384 M509	Y121 F129 Y135 F231 F236 G309 L375 H376 F379 M504

Gene Ontology

	Molecular Function
GO:0004497	monooxygenase activity
GO:0005506	iron ion binding
GO:0005515	protein binding
GO:0008398	sterol 14-demethylase activity
GO:0016491	oxidoreductase activity
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0020037	heme binding
GO:0046872	metal ion binding

	Biological Process
GO:0006696	ergosterol biosynthetic process
GO:0016126	sterol biosynthetic process

	Cellular Component
GO:0005783	endoplasmic reticulum
GO:0005789	endoplasmic reticulum membrane
GO:0016020	membrane

View graph for
Molecular Function

View graph for
Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:4lxj, PDBe:4lxj, PDBj:4lxj
PDBsum	4lxj
PubMed	24613931
UniProt	P10614\|CP51_YEAST Lanosterol 14-alpha demethylase CYP51 (Gene Name=ERG11)

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