Structure of PDB 4lwb Chain A

Receptor sequence

>4lwbA (length=362) Species: 224308 (Bacillus subtilis subsp. subtilis str. 168)

EEKEILWNEAKAFIAACYQELGKAAEVKDRLADIKSEIDLTGSYVHTKEE
LEHGAKMAWRNSNRCIGRLFWNSLNVIDRRDVRTKEEVRDALFHHIETAT
NNGKIRPTITIFPPEEKGEKQVEIWNHQLIRYAGYESDGERIGDPASCSL
TAACEELGWRGERTDFDLLPLIFRMKGDEQPVWYELPRSLVIEVPITHPD
IEAFSDLELKWYGVPIISDMKLEVGGIHYNAAPFNGWYMGTEIGARNLAD
EKRYDKLKKVASVIGIAADYNTDLWKDQALVELNKAVLHSYKKQGVSIVD
HHTAASQFKRFEEQAEEAGRKLTGDWTWLIPPISPAATHIFHRSYDNSIV
KPNYFYQDKPYE

3D structure

• PDB: 4lwb Structural and biological studies on bacterial nitric oxide synthase inhibitors.
• Chain: A
• Resolution: 2.15 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C66 R69 W238 E243
• Catalytic site (residue number reindexed from 1): C65 R68 W237 E242
• Enzyme Commision number: 1.14.14.47: nitric-oxide synthase (flavodoxin).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEM	A	W60 R65 C66 F235 G237 W238 E243 W329 Y355 Y357	W59 R64 C65 F234 G236 W237 E242 W328 Y354 Y356
BS02	QJ8	A	H128 I218 D220 F235 W238 E243 Y357	H127 I217 D219 F234 W237 E242 Y356	MOAD: Kd=4.4uM
BS03	H4B	A	R247 T328 W329	R246 T327 W328

Gene Ontology

Molecular Function

• GO:0004517 nitric-oxide synthase activity
• GO:0016491 oxidoreductase activity
• GO:0020037 heme binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006809 nitric oxide biosynthetic process

Cellular Component

• GO:0005575 cellular_component

External links

• PDB: RCSB:4lwb, PDBe:4lwb, PDBj:4lwb
• PDBsum: 4lwb
• PubMed: 24145412
• UniProt: O34453|NOSO_BACSU Nitric oxide synthase oxygenase (Gene Name=nos)