Structure of PDB 4lpc Chain A

Receptor sequence

>4lpcA (length=587) Species: 562 (Escherichia coli)

THLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWDGRRHPM
RLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPET
ASLICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYR
ELADQLVPYAKWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRD
DFRYFIDAAHAAGLNVILDWVPGHFPTDDFALAEFDGTNLYEHSTLIYNY
GRREVSNFLVGNALYWIERFGIDALRVDAVASMIYRGGRENLEAIEFLRN
TNRILGEQVSGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLD
YMKLDPVYRQYHHDKLTFGILYNYTENFVLPLSHDEVVHGKKSILDRMPG
DAWQKFANLRAYYGWMWAFPGKKLLFMGNEFAQGREWNHDASLDWHLLEG
GDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPYGFEWLVVDDKERSVLIF
VRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTDSMHYHGSNA
GNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE

3D structure

• PDB: 4lpc Crystal Structures of Escherichia coli Branching Enzyme in Complex with Linear Oligosaccharides.
• Chain: A
• Resolution: 2.39 Å

Enzymatic activity

• Enzyme Commision number: 2.4.1.18: 1,4-alpha-glucan branching enzyme.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	A	D585 H587	D444 H446
BS02	GLC	A	R255 S583 W586 H587	R139 S442 W445 H446
BS03	GLC	A	S467 R468 P469 Q470 G476 F477 W478	S326 R327 P328 Q329 G335 F336 W337
BS04	GLC	A	R468 N518	R327 N377
BS05	GLC	A	T508 I511 L512 W628	T367 I370 L371 W487
BS06	GLC	A	D505 T508 F509 L512	D364 T367 F368 L371

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0003844 1,4-alpha-glucan branching enzyme activity
• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0043169 cation binding

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0005978 glycogen biosynthetic process

External links

• PDB: RCSB:4lpc, PDBe:4lpc, PDBj:4lpc
• PDBsum: 4lpc
• PubMed: 26280198
• UniProt: P07762|GLGB_ECOLI 1,4-alpha-glucan branching enzyme GlgB (Gene Name=glgB)