Structure of PDB 4lmn Chain A

Receptor sequence
>4lmnA (length=292) Species: 9606 (Homo sapiens) [Search protein sequence]
MELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQ
IIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGR
IPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFG
VSGQLIDSMANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGR
YPIPPPDAKELELMFPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNK
CLIKNPAERADLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLN
3D structure
PDB4lmn Mechanism of MEK inhibition determines efficacy in mutant KRAS- versus BRAF-driven cancers.
ChainA
Resolution2.8 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D190 K192 S194 N195 D208 D217 T226
Catalytic site (residue number reindexed from 1) D130 K132 S134 N135 D148 D157 T166
Enzyme Commision number 2.7.12.2: mitogen-activated protein kinase kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ANP A A76 G77 V82 A95 K97 M143 E144 M146 Q153 K192 S194 N195 L197 D208 A16 G17 V22 A35 K37 M83 E84 M86 Q93 K132 S134 N135 L137 D148
BS02 MG A N195 D208 N135 D148
BS03 EUI A K97 L118 V127 I141 M143 D190 D208 F209 S212 L215 K37 L58 V67 I81 M83 D130 D148 F149 S152 L155 PDBbind-CN: -logKd/Ki=10.30,Ki=0.05nM
BindingDB: IC50=4.2nM
Gene Ontology
Molecular Function
GO:0004672 protein kinase activity
GO:0005524 ATP binding
Biological Process
GO:0006468 protein phosphorylation

View graph for
Molecular Function
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Biological Process
External links
PDB RCSB:4lmn, PDBe:4lmn, PDBj:4lmn
PDBsum4lmn
PubMed23934108
UniProtQ02750|MP2K1_HUMAN Dual specificity mitogen-activated protein kinase kinase 1 (Gene Name=MAP2K1)

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