Structure of PDB 4lhm Chain A

Receptor sequence
>4lhmA (length=440) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
LFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAMTIFFHD
MTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGP
MVAACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVG
VAIIGQTSSLAPADKRFYATRDITATVDSIPLITASILAKKLAEGLDALV
MDVKVGSGAFMPTYELSEALAEAIVGVANGAGVRTTALLTDMNQVLASSA
GNAVEVREAVQFLTGEYRNPRLFDVTMALCVEMLISGKLAKDDAEARAKL
QAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYLPTAMLTKAVYADTEGF
VSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQVDGQRPLA
VIHAKDENNWQEAAKAVKAAIKLADKAPESTPTVYRRISE
3D structure
PDB4lhm 3'-Azidothymidine in the active site of Escherichia coli thymidine phosphorylase: the peculiarity of the binding on the basis of X-ray study.
ChainA
Resolution1.52 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D83 K84 H85 S86 T123 D164 R171 S186 K190 K191
Catalytic site (residue number reindexed from 1) D83 K84 H85 S86 T123 D164 R171 S186 K190 K191
Enzyme Commision number 2.4.2.4: thymidine phosphorylase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 SO4 A K84 S86 S95 S113 T123 K84 S86 S95 S113 T123
BS02 SO4 A A175 V177 D178 A175 V177 D178
BS03 SO4 A R257 H403 R257 H403
BS04 SO4 A R234 K288 R234 K288
BS05 SO4 A R437 S439 R437 S439
BS06 AZZ A T87 Y168 R171 V177 S186 K190 F210 T87 Y168 R171 V177 S186 K190 F210 MOAD: Ki=0.68mM
PDBbind-CN: -logKd/Ki=3.39,Ki=0.41mM
Gene Ontology
Molecular Function
GO:0004645 1,4-alpha-oligoglucan phosphorylase activity
GO:0009032 thymidine phosphorylase activity
GO:0016154 pyrimidine-nucleoside phosphorylase activity
GO:0016757 glycosyltransferase activity
GO:0016763 pentosyltransferase activity
Biological Process
GO:0006206 pyrimidine nucleobase metabolic process
GO:0006213 pyrimidine nucleoside metabolic process
GO:0006974 DNA damage response
GO:0046104 thymidine metabolic process
Cellular Component
GO:0005829 cytosol
GO:0016020 membrane

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:4lhm, PDBe:4lhm, PDBj:4lhm
PDBsum4lhm
PubMed24699659
UniProtP07650|TYPH_ECOLI Thymidine phosphorylase (Gene Name=deoA)

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