Structure of PDB 4kqi Chain A

Receptor sequence

>4kqiA (length=340) Species: 99287 (Salmonella enterica subsp. enterica serovar Typhimurium str. LT2)

TLHALLRDIPAPDAEAMARAQQHIDGLLKPPGSLGRLETLAVQLAGMPGL
NGTPQVGEKAVLVMCADHGVWDEGVAVSPKIVTAIQAANMTRGTTGVCVL
AAQAGAKVHVIDVGIDAEPIPGVVNMRVARGCGNIAVGPAMSRLQAEALL
LEVSRYTCDLAQRGVTLFGVGELGMANTTPAAAMVSVFTGSDAKEVVGIG
ANLPPSRIDNKVDVVRRAIAINQPNPRDGIDVLSKVGGFDLVGMTGVMLG
AARCGLPVLLDGFLSYSAALAACQIAPAVRPYLIPSHFSAEKGARIALAH
LSMEPYLHMAMRLGAGSGAALAMPIVEAACAMFHNMGELA

3D structure

• PDB: 4kqi Dissecting cobamide diversity through structural and functional analyses of the base-activating CobT enzyme of Salmonella enterica.
• Chain: A
• Resolution: 1.4 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): E174 A317
• Catalytic site (residue number reindexed from 1): E172 A315
• Enzyme Commision number: 2.4.2.21: nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	RBZ	A	S80 E174 L175 G176 M177 A178 N179 T180 G202 A203 L315	S78 E172 L173 G174 M175 A176 N177 T178 G200 A201 L313
BS02	NIO	A	T180 G202 G264 F265 L266 S291 R314	T178 G200 G262 F263 L264 S289 R312

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0008939 nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity
• GO:0016757 glycosyltransferase activity

Biological Process

• GO:0009236 cobalamin biosynthetic process

External links

• PDB: RCSB:4kqi, PDBe:4kqi, PDBj:4kqi
• PDBsum: 4kqi
• PubMed: 24121107
• UniProt: Q05603|COBT_SALTY Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (Gene Name=cobT)