Structure of PDB 4kif Chain A

Receptor sequence
>4kifA (length=335) Species: 1912 (Streptomyces hygroscopicus) [Search protein sequence]
TEVSEAQARRAVADIFNSTLASSAIGAAWELGALDELRENGKLDVSDFAV
RHDLHEPAVVGMFTALASVGIVRREGATVVVGPYFDEANHHRSLFHWLNQ
GSGELFRRMPQVLPNENRTGKFYQRDAGAISYACREISERYFDPAFWAAV
DGLGYTPTTVADLGSGSGERLIQIARRFPGVRGLGVDIADGAIAMAEKEV
AAKGFGDQISFVRGDARTIDQVSARGEFAEVDLLTCFMMGHDFWPRENCV
QTLRKLRAAFPNVRRFLLGDATRTVGIPDRELPVFTLGFEFGHDMMGVYL
PTLDEWDGVFEEGGWRCVKKHAIDSLSVSVVFELE
3D structure
PDB4kif Structure and mechanism of a nonhaem-iron SAM-dependent C-methyltransferase and its engineering to a hydratase and an O-methyltransferase
ChainA
Resolution2.5 Å
3D
structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Enzyme Commision number 2.1.1.281: phenylpyruvate C(3)-methyltransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 PPY A R127 M240 H243 F287 F291 H295 R125 M238 H241 F285 F289 H293 PDBbind-CN: -logKd/Ki=4.84,Kd=14.6uM
BS02 FE A H243 H295 H241 H293
BS03 HF2 A D309 F312 C319 V320 K321 K322 D307 F310 C317 V318 K319 K320
BS04 PPY A G206 F207 D209 G204 F205 D207 PDBbind-CN: -logKd/Ki=4.84,Kd=14.6uM
BS05 CA A E106 R127 D128 E104 R125 D126
Gene Ontology
Molecular Function
GO:0008168 methyltransferase activity
Biological Process
GO:0009058 biosynthetic process
GO:0017000 antibiotic biosynthetic process
GO:0032259 methylation

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:4kif, PDBe:4kif, PDBj:4kif
PDBsum4kif
PubMed24914966
UniProtQ643C8|MPPJ_STRHY Phenylpyruvate C(3)-methyltransferase (Gene Name=mppJ)

[Back to BioLiP]