Structure of PDB 4k99 Chain A

Receptor sequence

>4k99A (length=354) Species: 10090 (Mus musculus)

KLKKVLDKLRLKRKDISEAAETVNKVVERLLRRMQKRESEFKGVEQLNTG
SYYEHVKISAPNEFDVMFKLEVPRIELQEYYETGAFYLVKFKGNPLSHFL
EGEVLSATKMLSKFRKIIKEEVKEIKDIDVSVEKEKPGSPAVTLLIRNPE
EISVDIILALESKGSWPISTKEGLPIQGWLGTKVRTNLRREPFYLVPKNA
KDGNSFQGETWRLSFSHTEKYILNNHGIEKTCCESSGAKCCRKECLKLMK
YLLEQLKKEFQELDAFCSYHVKTAIFHMWTQDPQDSQWDPRNLSSCFDKL
LAFFLECLRTEKLDHYFIPKFNLFSQELIDRKSKEFLSKKIEYERNNGFP
IFDK

3D structure

• PDB: 4k99 Cyclic [G(2',5')pA(3',5')p] is the metazoan second messenger produced by DNA-activated cyclic GMP-AMP synthase.
• Chain: A
• Resolution: 1.95 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.86: cyclic GMP-AMP synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	R158 L159 K160 R161 R180 H203 N376 E386 K395	R10 L11 K12 R13 R32 H55 N224 E234 K243
BS02	dna	A	R161 S165 A168 N196 Y200 K372	R13 S17 A20 N48 Y52 K220
BS03	ZN	A	H378 C385 C392	H226 C233 C240
BS04	MG	A	E211 D213	E63 D65
BS05	MG	A	E211 D213 D307	E63 D65 D155
BS06	GH3	A	G198 S199 E211 D213 R364 K402 C419 S420 Y421	G50 S51 E63 D65 R212 K250 C267 S268 Y269
BS07	GDO	A	M215 P292 D307 R364 S366 S368	M67 P140 D155 R212 S214 S216

External links

• PDB: RCSB:4k99, PDBe:4k99, PDBj:4k99
• PDBsum: 4k99
• PubMed: 23647843
• UniProt: Q8C6L5|CGAS_MOUSE Cyclic GMP-AMP synthase (Gene Name=Cgas)