Structure of PDB 4k4w Chain A

Receptor sequence

>4k4wA (length=462) Species: 12081 (Human poliovirus 1 Mahoney)

GEIQWMRPSKEVGYPIINAPSKTKLEPSAFHYVFEGVKEPAVLTKNDPRL
KTDFEEAIFSKYVGNKITEVDEYMKEAVDHYAGQLMSLDINTEQMCLEDA
MYGTDGLEALDLSTSAGYPYVAMGKKKRDILNKQTRDTKEMQKLLDTYGI
NLPLVTYVKDELRSKTKVEQGKSRLIEASSLNDSVAMRMAFGNLYAAFHK
NPGVITGSAVGCDPDLFWSKIPVLMEEKLFAFDYTGYDASLSPAWFEALK
MVLEKIGFGDRVDYIDYLNHSHHLYKNKTYCVKGGMPSGASGTSIFNSMI
NNLIIRTLLLKTYKGIDLDHLKMIAYGDDVIASYPHEVDASLLAQSGKDY
GLTMTPADKSATFETVTWENVTFLKRFFRADEKYPFLIHPVMPMKEIHES
IRWTKDPRNTQDHVRSLCLLAWHNGEEEYNKFLAKIRSVPIGRALDLPEY
STLYRRWLDSFG

3D structure

• PDB: 4k4w Structures of coxsackievirus, rhinovirus, and poliovirus polymerase elongation complexes solved by engineering RNA mediated crystal contacts.
• Chain: A
• Resolution: 2.69 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.4.22.28: picornain 3C.
  3.4.22.29: picornain 2A.
  3.6.1.15: nucleoside-triphosphate phosphatase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	N18 P20 K22 K24 T114 S115 K127 Y157 I176 A178 R188 V210 G211 C212 D213 G289 A290 Y326 K405 R408	N18 P20 K22 K24 T114 S115 K127 Y157 I176 A178 R188 V210 G211 C212 D213 G289 A290 Y326 K405 R408
BS02	rna	A	R128 K133 Y326 D329 S400 N409 D412 H413 S416	R128 K133 Y326 D329 S400 N409 D412 H413 S416

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:4k4w, PDBe:4k4w, PDBj:4k4w
• PDBsum: 4k4w
• PubMed: 23667424
• UniProt: P03300|POLG_POL1M Genome polyprotein