Structure of PDB 4k4g Chain A

Receptor sequence

>4k4gA (length=322) Species: 9606 (Homo sapiens)

TNHNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVTSYQEA
CSIPGIGKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKTA
QMWYQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQTV
QKAAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLLD
SLRQEGFLTDDLVSQEENGQQQKYLGVCRLPGPGRRHRRLDIIVVPYSEF
ACALLYFTGSAHFNRSMRALAKTKGMSLSEHALSTAVVPGRVLPTPTEKD
VFRLLGLPYREPAERDWLEHHH

3D structure

• PDB: 4k4g Structural basis for the binding and incorporation of nucleotide analogs with L-stereochemistry by human DNA polymerase lambda.
• Chain: A
• Resolution: 2.15 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D427 D429 D490
• Catalytic site (residue number reindexed from 1): D178 D180 D241
• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.
  4.2.99.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	W274 V462 S463 Q464 Q471 R514 R517 K521 L527 E529	W25 V213 S214 Q215 Q222 R265 R268 K272 L278 E280
BS02	dna	A	W342 G343 G345 K347 T348 D490 Y505	W93 G94 G96 K98 T99 D241 Y256
BS03	dna	A	Y267 W274 R275 Y279 G304 I305 G306 R308 M309 K312	Y18 W25 R26 Y30 G55 I56 G57 R59 M60 K63
BS04	1S0	A	R386 G416 S417 R420 C425 G426 D427 D429 Y505 F506 A510 N513	R137 G167 S168 R171 C176 G177 D178 D180 Y256 F257 A261 N264
BS05	CA	A	D427 D429	D178 D180
BS06	CA	A	D427 D429 D490	D178 D180 D241
BS07	CA	A	S339 I341 W342 A344	S90 I92 W93 A95
BS08	CA	A	C300 I302 P303 I305	C51 I53 P54 I56

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:4k4g, PDBe:4k4g, PDBj:4k4g
• PDBsum: 4k4g
• PubMed: 25015085
• UniProt: Q9UGP5|DPOLL_HUMAN DNA polymerase lambda (Gene Name=POLL)