Structure of PDB 4k39 Chain A

Receptor sequence

>4k39A (length=361) Species: 195103 (Clostridium perfringens ATCC 13124)

PPLSLLIKPASSGCNLKCTYCFYHKSYGIMRDEVLESMVKRVLNEANGHC
SFAFQGGEPTLAGLEFFEKLMELQRKHNYKNLKIYNSLQTNGTLIDESWA
KFLSENKFLVGLSMDGPKEIHNLNRKDCCGLDTFSKVERAAELFKKYKVE
FNILCVVTSNTARHVNKVYKYFKEKDFKFLQFINCLDPLYEEKGKYNYSL
KPKDYTKFLKNLFDFWYEDFLNGNRVSIRYFDGLLETILLGKSSSCGMNG
TCTCQFVVESDGSVYPCDFYVLDKWRLGNIQDMTMKELFETNKNHEFIKL
SFKVHEECKKCKWFRLCKGGCRRCRDSKEDSALELNYYCQSYKEFFEYAF
PRLINVANNIK

3D structure

• PDB: 4k39 X-ray structure of an AdoMet radical activase reveals an anaerobic solution for formylglycine posttranslational modification.
• Chain: A
• Resolution: 1.783 Å

Enzymatic activity

• Enzyme Commision number: 1.8.98.7: cysteine-type anaerobic sulfatase-maturating enzyme.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	Q64 L118 G120 E159 F160 N161 L163 F188 Q190 I192 R238 S253 S254 C255 Q264	Q55 L109 G111 E150 F151 N152 L154 F179 Q181 I183 R229 S244 S245 C246 Q255
BS02	SF4	A	C255 G256 C261 T262 Q264 C276 F278 C330 R331 R332	C246 G247 C252 T253 Q255 C267 F269 C321 R322 R323
BS03	SF4	A	C317 C320 F323 C326 C348 K352	C308 C311 F314 C317 C339 K343
BS04	SF4	A	C15 L17 C19 C22 G66 N100 R134	C14 L16 C18 C21 G57 N91 R125
BS05	SAM	A	Y21 F23 Y24 G65 G66 E67 Q98 T99 S122 R134 V165 I192 N193 C194 L195	Y20 F22 Y23 G56 G57 E58 Q89 T90 S113 R125 V156 I183 N184 C185 L186

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

External links

• PDB: RCSB:4k39, PDBe:4k39, PDBj:4k39
• PDBsum: 4k39
• PubMed: 23650368
• UniProt: Q0TTH1|ANSME_CLOP1 Cysteine-type anaerobic sulfatase-maturating enzyme (Gene Name=CPF_0616)