Structure of PDB 4juo Chain A

Receptor sequence

>4juoA (length=479) Species: 170187 (Streptococcus pneumoniae TIGR4)

NIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRILYSMNKDS
NTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEP
TVLPAAFPNLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKID
KLMEFLPGPDFPTGAIIQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKE
QIVITEIPYEINKANLVKKIDDVRVNNKVAGIAEVRDESDRDGLRIAIEL
KKDANTELVLNYLFKYTDLQINYNFNMVAIDNFTPRQVIVPILSSYIAHR
REVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALIRASENKADAKE
NLKVSYDFTEEQAEAIVTLQLYRLTNDVVVLQEEEAELREKIAMLAAIIG
DERTMYNLMKKELREVKKKFTPRLSSLED

3D structure

• PDB: 4juo Structure of an 'open' clamp type II topoisomerase-DNA complex provides a mechanism for DNA capture and transport.
• Chain: A
• Resolution: 6.53 Å

Enzymatic activity

• Enzyme Commision number: 5.6.2.2: DNA topoisomerase (ATP-hydrolyzing).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	R28 K38 H74 H76 S80 A84 R87 I170	R26 K36 H72 H74 S78 A82 R85 I168
BS02	dna	A	R117 Y118 I170 S171 G173 Y174 A175 N326	R115 Y116 I168 S169 G171 Y172 A173 N324
BS03	dna	A	R28 K38 R87	R26 K36 R85
BS04	dna	A	R117 Y118 I170 N326	R115 Y116 I168 N324

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
• GO:0005524 ATP binding

Biological Process

• GO:0006259 DNA metabolic process
• GO:0006265 DNA topological change

Cellular Component

• GO:0005694 chromosome

External links

• PDB: RCSB:4juo, PDBe:4juo, PDBj:4juo
• PDBsum: 4juo
• PubMed: 23965305
• UniProt: P72525|PARC_STRPN DNA topoisomerase 4 subunit A (Gene Name=parC)