Structure of PDB 4jkl Chain A

Receptor sequence

>4jklA (length=587) Species: 208435 (Streptococcus agalactiae 2603V/R)

NAMLYPLLTKTRNTYDLGGIWNFKLGEHNPNELLPSDEVMVIPTSFNDLM
VSKEKRDYIGDFWYEKVIEVPKVSEDEEMVLRFGSVTHQAKIYVDGVLVG
EHKGGFTPFEVLVPECKYNNEKIKVSICANNVLDYTTLPVGNYSEIIQED
GSIKKKVRENFDFFNYAGVHRPLKLMIRPKNHIFDITITSRLSDDLQSAD
LHFLVETNQKVDEVRISVFDEDNKLVGETKDSRLFLSDVHLWEVLNAYLY
TARVEIFVDNQLQDVYEENFGLREIEVTNGQFLLNRKPIYFKGFGKHEDT
FINGRGLNEAANLMDLNLLKDMGANSFRTSHYPYSEEMMRLADRMGVLVI
DEVPAVGLFQNNGTWNLMQTKAAHEQAIQELVKRDKNHPSVVMWVVANEP
ASHEAGAHDYFEPLVKLYKDLDPQKRPVTLVNILMATPDRDQVMDLVDVV
CLNRYYGWYVDHGDLTNAEVGIRKELLEWQDKFPDKPIIITEYGADTLPG
LHSTWNIPYTEEFQCDFYEMSHRVFDGIPNLVGEQVWNFADFETNLMILR
VQGNHKGLFSRNRQPKQVVKEFKKRWMTIPHYHNKKN

3D structure

• PDB: 4jkl Structure and Inhibition of Microbiome beta-Glucuronidases Essential to the Alleviation of Cancer Drug Toxicity.
• Chain: A
• Resolution: 2.288 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D160 H295 H329 V354 L356 E408 Y464 E501 K565
• Catalytic site (residue number reindexed from 1): D162 H297 H331 V356 L358 E399 Y455 E492 K556
• Enzyme Commision number: 3.2.1.31: beta-glucuronidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MG	A	R463 Y464 Y465	R454 Y455 Y456

Gene Ontology

Molecular Function

• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0004565 beta-galactosidase activity
• GO:0004566 beta-glucuronidase activity
• GO:0016798 hydrolase activity, acting on glycosyl bonds
• GO:0030246 carbohydrate binding

Biological Process

• GO:0005975 carbohydrate metabolic process

External links

• PDB: RCSB:4jkl, PDBe:4jkl, PDBj:4jkl
• PDBsum: 4jkl
• PubMed: 26364932
• UniProt: Q8E0N2|BGLR_STRA5 Beta-glucuronidase (Gene Name=SAG0698)