Structure of PDB 4jki Chain A

Receptor sequence

>4jkiA (length=557) Species: 264732 (Moorella thermoacetica ATCC 39073)

KVPSDIEIAQAAKMKPVMELARGLGIQEDEVELYGKYKAKISLDVYRRLK
DKPDGKLILVTAITPTPAGEGKTTTSVGLTDALARLGKRVMVCLREPSLG
PSFGIKGGAAGGGYAQVVPMEDINLHFTGDIHAVTYAHNLLAAMVDNHLQ
QGNVLNIDPRTITWRRVIDLNDRALRNIVIGLGGKANGVPRETGFDISVA
SEVMACLCLASDLMDLKERFSRIVVGYTYDGKPVTAGDLEAQGSMALLMK
DAIKPNLVQTLENTPAFIHGGPFANIAHGCNSIIATKTALKLADYVVTEA
GFGADLGAEKFYDVKCRYAGFKPDATVIVATVRALKMHGGVPKSDLATEN
LEALREGFANLEKHIENIGKFGVPAVVAINAFPTDTEAELNLLYELCAKA
GAEVALSEVWAKGGEGGLELARKVLQTLESRPSNFHVLYNLDLSIKDKIA
KIATEIYGADGVNYTAEADKAIQRYESLGYGNLPVVMAKTQYSFSDDMTK
LGRPRNFTITVREVRLSAGAGFIVPITGAIMTMPGLPKRPAACNIDIDAD
GVITGLF

3D structure

• PDB: 4jki Mechanism of N10-formyltetrahydrofolate synthetase derived from complexes with intermediates and inhibitors.
• Chain: A
• Resolution: 2.6 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): K74 T76 R97 K108 A276 F304 F384 W412
• Catalytic site (residue number reindexed from 1): K72 T74 R95 K106 A274 F302 F382 W410
• Enzyme Commision number: 6.3.4.3: formate--tetrahydrofolate ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZD9	A	T76 G110 A111 G113 N382 A383 W412	T74 G108 A109 G111 N380 A381 W410
BS02	XPO	A	G73 K74 T75 T76	G71 K72 T73 T74
BS03	ADP	A	G73 K74 T76 N382 W412	G71 K72 T74 N380 W410

Gene Ontology

Molecular Function

• GO:0004329 formate-tetrahydrofolate ligase activity
• GO:0005524 ATP binding
• GO:0016874 ligase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0035999 tetrahydrofolate interconversion

External links

• PDB: RCSB:4jki, PDBe:4jki, PDBj:4jki
• PDBsum: 4jki
• PubMed: 22109967
• UniProt: Q2RM91|FTHS_MOOTA Formate--tetrahydrofolate ligase (Gene Name=fhs)