Structure of PDB 4jjz Chain A

Receptor sequence

>4jjzA (length=554) Species: 264732 (Moorella thermoacetica ATCC 39073)

PSDIEIAQAAKMKPVMELARGLGIQEDEVELYGKYKAKISLDVYRRLKDK
PDGKLILVTAITPTPAGEGKTTTSVGLTDALARLGKRVMVCLREPSLGPS
FGIKGGAAGGGYAQVVPMEDINLHFTGDIHAVTYAHNLLAAMVDNHLQQG
NVLNIDPRTITWRRVIDLNDRALRNIVIGLGGKANGVPRETGFDISVASE
VMACLCLASDLMDLKERFSRIVVGYTYDGKPVTAGDLEAQGSMALLMKDA
IKPNLVQTLENTPAFIHGGPFANIAHGCNSIIATKTALKLADYVVTEAGF
GADLGAEKFYDVKCRYAGFKPDATVIVATVRALKMHGGVPKSDLATENLE
ALREGFANLEKHIENIGKFGVPAVVAINAFPTDTEAELNLLYELCAKAGA
EVALSEVWAKGGEGGLELARKVLQTLESRPSNFHVLYNLDLSIKDKIAKI
ATEIYGADGVNYTAEADKAIQRYESLGYGNLPVVMAKTQYSFSDDMTKLG
RPRNFTITVREVRLSAGAGFIVPITGAIMTMPGLPKRPAACNIDIDADGV
ITGL

3D structure

• PDB: 4jjz Mechanism of N10-formyltetrahydrofolate synthetase derived from complexes with intermediates and inhibitors.
• Chain: A
• Resolution: 2.5 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): K74 T76 R97 K108 A276 F304 F384 W412
• Catalytic site (residue number reindexed from 1): K70 T72 R93 K104 A272 F300 F380 W408
• Enzyme Commision number: 6.3.4.3: formate--tetrahydrofolate ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ADP	A	A70 E72 G73 K74 T75 T76 G113 N382 F384 P385 W412	A66 E68 G69 K70 T71 T72 G109 N378 F380 P381 W408
BS02	XPO	A	K74 R97 F275 A276 F304	K70 R93 F271 A272 F300
BS03	MG	A	T75 E301	T71 E297

Gene Ontology

Molecular Function

• GO:0004329 formate-tetrahydrofolate ligase activity
• GO:0005524 ATP binding
• GO:0016874 ligase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0035999 tetrahydrofolate interconversion

External links

• PDB: RCSB:4jjz, PDBe:4jjz, PDBj:4jjz
• PDBsum: 4jjz
• PubMed: 22109967
• UniProt: Q2RM91|FTHS_MOOTA Formate--tetrahydrofolate ligase (Gene Name=fhs)