Structure of PDB 4jbt Chain A

Receptor sequence

>4jbtA (length=407) Species: 247156 (Nocardia farcinica IFM 10152)

CPHSDTLTIDPMITDLAGETSRLRAAGPLTRIDLLGVPALAVTGHTLARQ
LLTDTRLVKDINAWSLWQSGTVTRQWPLIGMIDVDRSMFTVDGPEHRRLR
IKTTQALTRRRLDALKPTIERYVAELLDDLERAGADGAVVDLKSVFAYPL
PMRVISALMGVPSEDQEQLLTWYKAFFSILTPQDERLRVIDEMHGYFTEM
VRRKTAEPGDDLTSALIYATDGETPLTEEEVIGNLQALVAAGHETTVSLI
LTAVRALLSHPEQLRLVRDGEIGWETAIEETLRWDGPVIHLLMRFATEDI
DLGDAVIPRGEGVVMSYRAIGRDITVHGADADDFDITRATAARHISFGHG
PHICPGAALARLEAAIALPALFTRFPHLHPALPLDQIPNLPVLTQNDLSH
FPIHLGR

3D structure

• PDB: 4jbt Enzyme-substrate complex structures of CYP154C5 shed light on its mode of highly selective steroid hydroxylation.
• Chain: A
• Resolution: 2.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): S181 A244 E247 T248 T249 V291 C357 P358 G359 E366 Q398
• Catalytic site (residue number reindexed from 1): S178 A241 E244 T245 T246 V288 C354 P355 G356 E363 Q395

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ASD	A	M84 F179 A240 A244	M81 F176 A237 A241
BS02	HEM	A	M91 H99 R103 A244 G245 T248 T249 P290 L294 R297 Y320 S349 F350 H355 C357 P358 G359 A363	M88 H96 R100 A241 G242 T245 T246 P287 L291 R294 Y317 S346 F347 H352 C354 P355 G356 A360
BS03	MG	A	H6 D8	H3 D5

Gene Ontology

Molecular Function

• GO:0004497 monooxygenase activity
• GO:0005506 iron ion binding
• GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
• GO:0020037 heme binding
• GO:0046872 metal ion binding

External links

• PDB: RCSB:4jbt, PDBe:4jbt, PDBj:4jbt
• PDBsum: 4jbt
• PubMed: 25372679
• UniProt: Q5YNS8