Structure of PDB 4jal Chain A

Receptor sequence
>4jalA (length=156) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
MALNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGL
DYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMF
GPETRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLG
YPGAVL
3D structure
PDB4jal The tRNA recognition mechanism of the minimalist SPOUT methyltransferase, TrmL
ChainA
Resolution2.0 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.1.1.207: tRNA (cytidine(34)-2'-O)-methyltransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 SAH A L78 T79 T80 G100 E102 R121 I122 M124 M131 N132 L133 L79 T80 T81 G101 E103 R122 I123 M125 M132 N133 L134 PDBbind-CN: -logKd/Ki=4.60,Kd=25uM
Gene Ontology
Molecular Function
GO:0003723 RNA binding
GO:0008168 methyltransferase activity
GO:0008173 RNA methyltransferase activity
GO:0008175 tRNA methyltransferase activity
GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
GO:0042803 protein homodimerization activity
GO:0141098 tRNA (cytidine(34)-2'-O)-methyltransferase activity
GO:0141102 tRNA (5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase activity
Biological Process
GO:0001510 RNA methylation
GO:0002131 wobble position cytosine ribose methylation
GO:0002132 wobble position uridine ribose methylation
GO:0006396 RNA processing
GO:0008033 tRNA processing
GO:0030488 tRNA methylation
GO:0032259 methylation
Cellular Component
GO:0005737 cytoplasm

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Cellular Component
External links
PDB RCSB:4jal, PDBe:4jal, PDBj:4jal
PDBsum4jal
PubMed23804755
UniProtP0AGJ7|TRML_ECOLI tRNA (cytidine(34)-2'-O)-methyltransferase (Gene Name=trmL)

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