Structure of PDB 4j9m Chain A

Receptor sequence

>4j9mA (length=429) Species: 9606 (Homo sapiens)

GPHMATGQDRVVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKGGGI
IAVSYEARAFGVTRSMWADDAKKLCPDLLLAQVRESRGKANLTKYREASV
EVMEIMSRFAVIERASIDEAYVDLTSAVQERLQKLQGQPISADLLPSTYI
EGLPQGTVQKEGMRKQGLFQWLDSLQIDNLTSPDLQLTVGAVIVEEMRAA
IERETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQMPIR
KIRSLGGKLGASVIEILGIEYMGELTQFTESQLQSHFGEKNGSWLYAMCR
GIEHDPVKPRQLPKTIGCSKNFPGKTALATREQVQWWLLQLAQELEERLT
KDRNDNDRVATQLVVSIRVQGDKRLSSLRRCCALTRYDAHKMSHDAFTVI
KNCNTSGIQTEWSPPLTMLFLCATKFSAS

3D structure

• PDB: 4j9m Mechanism of somatic hypermutation at the WA motif by human DNA polymerase eta.
• Chain: A
• Resolution: 2.25 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	Q38 Y39 W64 K86 R93 K293 P316 K317 T318 G320 C321 S322 K323 N324 P326 R351	Q41 Y42 W67 K89 R96 K290 P313 K314 T315 G317 C318 S319 K320 N321 P323 R348
BS02	dna	A	R61 S257 G259 G260 K261 L262 Q365 R382	R64 S254 G256 G257 K258 L259 Q362 R379
BS03	MG	A	D13 M14 D115	D16 M17 D118
BS04	MG	A	D13 D115 E116	D16 D118 E119
BS05	XG4	A	D13 M14 D15 C16 F17 F18 I48 Y52 R55 R61 D115 K231	D16 M17 D18 C19 F20 F21 I51 Y55 R58 R64 D118 K228

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:4j9m, PDBe:4j9m, PDBj:4j9m
• PDBsum: 4j9m
• PubMed: 23630267
• UniProt: Q9Y253|POLH_HUMAN DNA polymerase eta (Gene Name=POLH)