Structure of PDB 4j3v Chain A

Receptor sequence
>4j3vA (length=872) Species: 4513 (Hordeum vulgare) [Search protein sequence]
FMPDARAYWVTSDLIAWNVGELEAQSVCLYASRAAAMSLSGGIQGYDSKV
ELQPESAGLPETVTQKFPFISSYRAFRVPSSVDVASLVKCQLVVASVDVT
GLQLPGVLDDMFAYTGPLGAVFSEDSVSLHLWAPTAQGVSVCFFDGPAGP
ALETVQLKESNGVWSVTGPREWENRYYLYEVDVYHPTKAQVLKCLAGDPY
ARSLSANGARTWLVDINNETLKPASWDELADEKPKLDSFSDITIYELHIR
DFSAHDGTVDSDSRGGFRAFAYQASAGMEHLRKLSDAGLTHVHLLPSFHF
AGVDDIKSNWKFVDECELATFPPGSDMQQAAVVAIQEEDPYNWGYNPVLW
GVPKGSYASDPDGPSRIIEYRQMVQALNRIGLRVVMDVVYNHLDSSGPCG
ISSVLDKIVPGYYVRRDTNGQIENSAAMNNTASEHFMVDRLIVDDLLNWA
VNYKVDGFRFDLMGHIMKRTMMRAKSALQSLTTDAHGVDGSKIYLYGEGW
DFAEVARNQRGINGSQLNMSGTGIGSFNDRIRDAINGGNPFGNPLQQGFN
TGLFLEPNGFYQGNEADTRRSLATYADQIQIGLAGNLRDYVLISHTGEAK
KGSEIHTFDGLPVGYTASPIETINYVSAHDNETLFDVISVKTPMILSVDE
RCRINHLASSMMALSQGIPFFHAGDEILRSKSIDRDSYNSGDWFNKLDFT
YETNNWGVGLPPSEKNEDNWPLMKPRLENPSFKPAKGHILAALDSFVDIL
KIRYSSPLFRLSTANDIKQRVRFHNTGPSLVPGVIVMGIEDARGESPEMA
QLDTNFSYVVTVFNVCPHEVSMDIPALASMGFELHPVQVNSSDTLVRKSA
YEAATGRFTVPGRTVSVFVEPR
3D structure
PDB4j3v Oligosaccharide and substrate binding in the starch debranching enzyme barley limit dextrinase
ChainA
Resolution1.45 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) N219 H311 F312 K366 D473 E510 D642
Catalytic site (residue number reindexed from 1) N207 H299 F300 K354 D461 E498 D630
Enzyme Commision number 3.2.1.41: pullulanase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 GLC A D541 R544 D529 R532
BS02 GLC A W512 D642 N643 W500 D630 N631
BS03 GLC A W355 Y357 D473 E510 H641 D642 W343 Y345 D461 E498 H629 D630
BS04 GLC A N358 D698 Y700 N346 D686 Y688
BS05 SGD A Y700 K727 Y688 K715
BS06 GLC A D406 M440 D394 M428
BS07 GLC A N436 A439 M440 N424 A427 M428
BS08 CA A Q348 D351 Y353 N701 Q336 D339 Y341 N689
Gene Ontology
Molecular Function
GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
GO:0046872 metal ion binding
GO:0051060 pullulanase activity
Biological Process
GO:0000272 polysaccharide catabolic process
GO:0005975 carbohydrate metabolic process

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Molecular Function
View graph for
Biological Process
External links
PDB RCSB:4j3v, PDBe:4j3v, PDBj:4j3v
PDBsum4j3v
PubMed25562209
UniProtQ9FYY0

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