Structure of PDB 4j3s Chain A

Receptor sequence
>4j3sA (length=854) Species: 4513 (Hordeum vulgare) [Search protein sequence]
PDARAYWVTSDLIAWNVGSVCLYASRAAAMQGYDSKVELQPESAGLPETV
TQKFPFISSYRAFRVPSSVDVASLVKCQLVVASFVDVTGLQLPGVLDDMF
AYTGPLGAVFSEDSVSLHLWAPTAQGVSVCFFDGPAGPALETVQLKESNG
VWSVTGPREWENRYYLYEVDVYHPTKAQVLKCLAGDPYARSLSANGARTW
LVDINNETLKPASWDELADEKPKLDSFSDITIYELHIRDFSAHDGTVDSD
SRGGFRAFAYQASAGMEHLRKLSDAGLTHVHLLPSFHFAGVDDIKSNWKF
VDECELATFPPGSDMQQAAVVAIQEEDPYNWGYNPVLWGVPKGSYASDPD
GPSRIIEYRQMVQALNRIGLRVVMDVVYNHLDSSGPCGISSVLDKIVPGY
YVRRDTNGQIENSAAMNNTASEHFMVDRLIVDDLLNWAVNYKVDGFRFDL
MGHIMKRTMMRAKSALQSLTTDAHGVDGSKIYLYGEGWDFAEVARNQRGI
NGSQLNMSGTGIGSFNDRIRDAINGGNPFGNPLQQGFNTGLFLEPNGFDT
RRSLATYADQIQIGLAGNLRDYVLISHTGEAKKGSEIHTFDGLPVGYTAS
PIETINYVSAHDNETLFDVISVKTPMILSVDERCRINHLASSMMALSQGI
PFFHAGDEILRSKSIDRDSYNSGDWFNKLDFTYETNNWGVGLPPSEKNED
NWPLMKPRLENPSFKPAKGHILAALDSFVDILKIRYSSPLFRLSTANDIK
QRVRFHNTGPSLVPGVIVMGIEDARGESPEMAQLDTNFSYVVTVFNVCPH
EVSMDIPALASMGFELHPVQVNSSDTLVRKSAYEAATGRFTVPGRTVSVF
VEPR
3D structure
PDB4j3s Oligosaccharide and substrate binding in the starch debranching enzyme barley limit dextrinase
ChainA
Resolution1.75 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) N219 H311 F312 K366 D473 E510 D642
Catalytic site (residue number reindexed from 1) N195 H287 F288 K342 D449 E486 D612
Enzyme Commision number 3.2.1.41: pullulanase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 GLC A D541 R544 D517 R520
BS02 GLC A W512 D642 N643 W488 D612 N613
BS03 GLC A Y357 N358 D698 Y700 Y333 N334 D668 Y670
BS04 GLC A M440 Y700 K727 M416 Y670 K697
BS05 GLC A K247 S297 G393 K223 S273 G369
BS06 GLC A R294 R391 R270 R367
BS07 CA A Q348 D351 Y353 N701 Q324 D327 Y329 N671
Gene Ontology
Molecular Function
GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
GO:0046872 metal ion binding
GO:0051060 pullulanase activity
Biological Process
GO:0000272 polysaccharide catabolic process
GO:0005975 carbohydrate metabolic process

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:4j3s, PDBe:4j3s, PDBj:4j3s
PDBsum4j3s
PubMed25562209
UniProtQ9FYY0

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