Structure of PDB 4itx Chain A

Receptor sequence
>4itxA (length=391) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
KLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKHATRNRANGEL
FYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFIEQGDHV
LMTNTAYESSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLE
SPGSITMEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVS
IQAATKYLVGHSDAMIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSR
GLRTLGVRLRQHHESSLKVAEWLAEHPQVARVNHPALPGSKGHEFWKRDF
TGSSGLFSFVLKKKLNNEELANYLDNFSLFSMAYSWGGYESLILANQPEH
IAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFARIV
3D structure
PDB4itx Mechanistic and Evolutionary Insights from the Reciprocal Promiscuity of Two Pyridoxal Phosphate-dependent Enzymes.
ChainA
Resolution1.61 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R58 Y111 D185 K210
Catalytic site (residue number reindexed from 1) R54 Y107 D181 K206
Enzyme Commision number 4.4.1.13: cysteine-S-conjugate beta-lyase.
4.4.1.28: L-cysteine desulfidase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 IN5 A C85 G86 A87 Y111 D185 A207 T209 K210 M219 Y338 S339 W340 R372 C81 G82 A83 Y107 D181 A203 T205 K206 M215 Y334 S335 W336 R368
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0008784 alanine racemase activity
GO:0016829 lyase activity
GO:0030170 pyridoxal phosphate binding
GO:0042802 identical protein binding
GO:0047804 cysteine-S-conjugate beta-lyase activity
GO:0080146 L-cysteine desulfhydrase activity
Biological Process
GO:0006520 amino acid metabolic process
GO:0009086 methionine biosynthetic process
GO:0019346 transsulfuration
GO:0019450 L-cysteine catabolic process to pyruvate
GO:0051289 protein homotetramerization
Cellular Component
GO:0005737 cytoplasm
GO:0032991 protein-containing complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:4itx, PDBe:4itx, PDBj:4itx
PDBsum4itx
PubMed27474741
UniProtP06721|METC_ECOLI Cystathionine beta-lyase MetC (Gene Name=metC)

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