Structure of PDB 4iok Chain A

Receptor sequence

>4iokA (length=557) Species: 264732 (Moorella thermoacetica ATCC 39073)

KVPSDIEIAQAAKMKPVMELARGLGIQEDEVELYGKYKAKISLDVYRRLK
DKPDGKLILVTAITPTPAGEGKTTTSVGLTDALARLGKRVMVCLREPSLG
PSFGIKGGAAGGGYAQVVPMEDINLHFTGDIHAVTYAHNLLAAMVDNHLQ
QGNVLNIDPRTITWRRVIDLNDRALRNIVIGLGGKANGVPRETGFDISVA
SEVMACLCLASDLMDLKERFSRIVVGYTYDGKPVTAGDLEAQGSMALLMK
DAIKPNLVQTLENTPAFIHGGPFANIAHGCNSIIATKTALKLADYVVTEA
GFGADLGAEKFYDVKCRYAGFKPDATVIVATVRALKMHGGVPKSDLATEN
LEALREGFANLEKHIENIGKFGVPAVVAINAFPTDTEAELNLLYELCAKA
GAEVALSEVWAKGGEGGLELARKVLQTLESRPSNFHVLYNLDLSIKDKIA
KIATEIYGADGVNYTAEADKAIQRYESLGYGNLPVVMAKTQYSFSDDMTK
LGRPRNFTITVREVRLSAGAGFIVPITGAIMTMPGLPKRPAACNIDIDAD
GVITGLF

3D structure

• PDB: 4iok Time passes yet errors remain: Comments on the structure of N(10) -formyltetrahydrofolate synthetase.
• Chain: A
• Resolution: 2.497 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): K74 T76 R97 K108 A276 F304 F384 W412
• Catalytic site (residue number reindexed from 1): K72 T74 R95 K106 A274 F302 F382 W410
• Enzyme Commision number: 6.3.4.3: formate--tetrahydrofolate ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ADP	A	A70 G73 K74 T75 T76 G113 N382 A383 F384 P385 W412	A68 G71 K72 T73 T74 G111 N380 A381 F382 P383 W410
BS02	XPO	A	K74 R97 G303 F304	K72 R95 G301 F302
BS03	MG	A	T75 E301	T73 E299

Gene Ontology

Molecular Function

• GO:0004329 formate-tetrahydrofolate ligase activity
• GO:0005524 ATP binding
• GO:0016874 ligase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0035999 tetrahydrofolate interconversion

External links

• PDB: RCSB:4iok, PDBe:4iok, PDBj:4iok
• PDBsum: 4iok
• PubMed: 23533144
• UniProt: Q2RM91|FTHS_MOOTA Formate--tetrahydrofolate ligase (Gene Name=fhs)