BioLiP

PDB

4iay Insights into the Phosphoryl Transfer Catalyzed by cAMP-Dependent Protein Kinase: An X-ray Crystallographic Study of Complexes with Various Metals and Peptide Substrate SP20.

Chain

Resolution

2.0 Å

3D
structure

Catalytic site residues are labeled in the structure
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Site
#

Ligand

Ligand
chain

Binding residues on receptor
(original residue number in PDB)

Binding residues on receptor
(residue number reindexed from 1)

Binding affinity

BS01

peptide

S53 E127 F129 R133 E170 F187 L198 G200 T201 E203 E230 Y235 P236 F239 D241

S39 E113 F115 R119 E156 F173 L184 G186 T187 E189 E216 Y221 P222 F225 D227

BS02

ADP

V57 A70 K72 V123 E127 L173 D184 F327

V43 A56 K58 V109 E113 L159 D170 F313

Molecular Function

GO:0000287

magnesium ion binding

GO:0004672

protein kinase activity

GO:0004674

protein serine/threonine kinase activity

GO:0004679

AMP-activated protein kinase activity

GO:0004691

cAMP-dependent protein kinase activity

GO:0004712

protein serine/threonine/tyrosine kinase activity

GO:0005515

protein binding

GO:0005524

ATP binding

GO:0019901

protein kinase binding

GO:0019904

protein domain specific binding

GO:0030145

manganese ion binding

GO:0031625

ubiquitin protein ligase binding

GO:0034237

protein kinase A regulatory subunit binding

GO:0106310

protein serine kinase activity

	Biological Process
GO:0000122	negative regulation of transcription by RNA polymerase II
GO:0001707	mesoderm formation
GO:0001843	neural tube closure
GO:0006397	mRNA processing
GO:0006468	protein phosphorylation
GO:0006611	protein export from nucleus
GO:0007189	adenylate cyclase-activating G protein-coupled receptor signaling pathway
GO:0007193	adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
GO:0016310	phosphorylation
GO:0018105	peptidyl-serine phosphorylation
GO:0032024	positive regulation of insulin secretion
GO:0034605	cellular response to heat
GO:0045542	positive regulation of cholesterol biosynthetic process
GO:0045667	regulation of osteoblast differentiation
GO:0045722	positive regulation of gluconeogenesis
GO:0045879	negative regulation of smoothened signaling pathway
GO:0046827	positive regulation of protein export from nucleus
GO:0048240	sperm capacitation
GO:0050804	modulation of chemical synaptic transmission
GO:0051726	regulation of cell cycle
GO:0061136	regulation of proteasomal protein catabolic process
GO:0070417	cellular response to cold
GO:0070613	regulation of protein processing
GO:0071333	cellular response to glucose stimulus
GO:0071374	cellular response to parathyroid hormone stimulus
GO:0071377	cellular response to glucagon stimulus
GO:0099170	postsynaptic modulation of chemical synaptic transmission
GO:0141156	cAMP/PKA signal transduction
GO:1904262	negative regulation of TORC1 signaling
GO:1904539	negative regulation of glycolytic process through fructose-6-phosphate
GO:1990044	protein localization to lipid droplet
GO:2000810	regulation of bicellular tight junction assembly

Biological Process

GO:0000122

negative regulation of transcription by RNA polymerase II

mesoderm formation

neural tube closure

mRNA processing

protein phosphorylation

GO:0006611

protein export from nucleus

GO:0007189

adenylate cyclase-activating G protein-coupled receptor signaling pathway

GO:0007193

adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway

GO:0016310

phosphorylation

GO:0018105

peptidyl-serine phosphorylation

GO:0032024

positive regulation of insulin secretion

GO:0034605

cellular response to heat

GO:0045542

positive regulation of cholesterol biosynthetic process

GO:0045667

regulation of osteoblast differentiation

GO:0045722

positive regulation of gluconeogenesis

GO:0045879

negative regulation of smoothened signaling pathway

GO:0046827

positive regulation of protein export from nucleus

GO:0048240

sperm capacitation

GO:0050804

modulation of chemical synaptic transmission

GO:0051726

regulation of cell cycle

GO:0061136

regulation of proteasomal protein catabolic process

GO:0070417

cellular response to cold

GO:0070613

regulation of protein processing

GO:0071333

cellular response to glucose stimulus

GO:0071374

cellular response to parathyroid hormone stimulus

GO:0071377

cellular response to glucagon stimulus

GO:0099170

postsynaptic modulation of chemical synaptic transmission

GO:0141156

cAMP/PKA signal transduction

GO:1904262

negative regulation of TORC1 signaling

GO:1904539

negative regulation of glycolytic process through fructose-6-phosphate

GO:1990044

protein localization to lipid droplet

GO:2000810

regulation of bicellular tight junction assembly

	Cellular Component
GO:0001669	acrosomal vesicle
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005737	cytoplasm
GO:0005739	mitochondrion
GO:0005813	centrosome
GO:0005829	cytosol
GO:0005886	plasma membrane
GO:0005930	axoneme
GO:0005952	cAMP-dependent protein kinase complex
GO:0016607	nuclear speck
GO:0031410	cytoplasmic vesicle
GO:0031514	motile cilium
GO:0031594	neuromuscular junction
GO:0036126	sperm flagellum
GO:0044853	plasma membrane raft
GO:0048471	perinuclear region of cytoplasm
GO:0097546	ciliary base
GO:0098794	postsynapse
GO:0098978	glutamatergic synapse

Cellular Component

acrosomal vesicle

nucleus

nucleoplasm

cytoplasm

mitochondrion

centrosome

cytosol

plasma membrane

axoneme

cAMP-dependent protein kinase complex

nuclear speck

cytoplasmic vesicle

motile cilium

neuromuscular junction

GO:0036126

sperm flagellum

GO:0044853

plasma membrane raft

GO:0048471

perinuclear region of cytoplasm

GO:0097546

ciliary base

GO:0098794

postsynapse

GO:0098978

glutamatergic synapse

PDB	RCSB:4iay, PDBe:4iay, PDBj:4iay
PDBsum	4iay
PubMed	23672593
UniProt	P05132\|KAPCA_MOUSE cAMP-dependent protein kinase catalytic subunit alpha (Gene Name=Prkaca)

Structure of PDB 4iay Chain A