Structure of PDB 4hym Chain A

Receptor sequence
>4hymA (length=361) Species: 376619 (Francisella tularensis subsp. holarctica LVS) [Search protein sequence]
KSIEVLTGLDPVKKRPGMYTNIENPNHLIQEIIDNSVDEVLAGFASKINI
TLYEDNSIEVADDGRGMPVDIHPEHKMSGIELIMTKLHSGGKFSNGGLHG
VGVSVVNALSTRLEAEIKRDGNVYHIVFEDGFKTKDLEIIDNVGKKNTGT
KIRFWPNKKYFDDIKVNFKALKNLLEAKAILCKALTIKYSNEIKKEKLTW
HFETGLKGYLDHKLEAETLPAEPFIIDNFSNGDSYLDAVFCWCEDPSESI
KNSYVNLIPTPQDGTHVTGLKNGIYDAIKAYIEKNSNIKITANDSFAQLN
YVISVKITNPQFAGQTKEKLSNKDVTNFVATAVKDLLTIWLNQNPDEARQ
IVENISKVAQK
3D structure
PDB4hym Pyrrolopyrimidine inhibitors of DNA gyrase B (GyrB) and topoisomerase IV (ParE). Part I: Structure guided discovery and optimization of dual targeting agents with potent, broad-spectrum enzymatic activity.
ChainA
Resolution1.9 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 5.6.2.2: DNA topoisomerase (ATP-hydrolyzing).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 CJC A I38 N41 S42 E45 D68 G72 M73 I89 T162 I164 I32 N35 S36 E39 D62 G66 M67 I83 T150 I152 MOAD: Ki=6nM
PDBbind-CN: -logKd/Ki=8.22,Ki=6nM
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
GO:0005524 ATP binding
Biological Process
GO:0006265 DNA topological change
Cellular Component
GO:0005694 chromosome

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:4hym, PDBe:4hym, PDBj:4hym
PDBsum4hym
PubMed23352267
UniProtA0A0J9WZF0|PARE_FRATH DNA topoisomerase 4 subunit B (Gene Name=parE)

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