Structure of PDB 4hom Chain A

Receptor sequence

>4homA (length=902) Species: 9823 (Sus scrofa)

QSKPWNRYRLPTTLLPDSYNVTLRPYLTPNADGLYIFKGKSIVRFLCQEP
TDVIIIHSKKLNYTTQGHMVVLRGVGDSQVPEIDRTELVELTEYLVVHLK
GSLQPGHMYEMESEFQGELADDLAGFYRSEYMEGNVKKVLATTQMQSTDA
RKSFPCFDEPAMKATFNITLIHPNNLTALSNMPPKGSSTPLAEDPNWSVT
EFETTPVMSTYLLAYIVSEFQSVNETAQNGVLIRIWARPNAIAEGHGMYA
LNVTGPILNFFANHYNTSYPLPKSDQIALPDFNAGAMENWGLVTYRENAL
LFDPQSSSISNKERVVTVIAHELAHQWFGNLVTLAWWNDLWLNEGFASYV
EYLGADHAEPTWNLKDLIVPGDVYRVMAVDALASSHPLTTPAEEVNTPAQ
ISEMFDSISYSKGASVIRMLSNFLTEDLFKEGLASYLHAFAYQNTTYLDL
WEHLQKAVDAQTSIRLPDTVRAIMDRWTLQMGFPVITVDTKTGNISQKHF
LLDSESNVTRSSAFDYLWIVPISSIKNGVMQDHYWLRDVSQAQNDLFKTA
SDDWVLLNVNVTGYFQVNYDEDNWRMIQHQLQTNLSVIPVINRAQVIYDS
FNLATAHMVPVTLALDNTLFLNGEKEYMPWQAALSSLSYFSLMFDRSEVY
GPMKKYLRKQVEPLFQHFETLTKNWTERPENLMDQYSEINAISTACSNGL
PQCENLAKTLFDQWMSDPENNPIHPNLRSTIYCNAIAQGGQDQWDFAWGQ
LQQAQLVNEADKLRSALACSNEVWLLNRYLGYTLNPDLIRKQDATSTINS
IASNVIGQPLAWDFVQSNWKKLFQDYGGGSFSFSNLIQGVTRRFSSEFEL
QQLEQFKKNNMDVGFGSGTRALEQALEKTKANIKWVKENKEVVLNWFIEH
SS

3D structure

• PDB: 4hom Structural basis for multifunctional roles of mammalian aminopeptidase N.
• Chain: A
• Resolution: 1.9 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): E350 H383 E384 H387 E406 S464 Y472
• Catalytic site (residue number reindexed from 1): E288 H321 E322 H325 E344 S402 Y410
• Enzyme Commision number: 3.4.11.2: membrane alanyl aminopeptidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	A346 G347 A348 E375 V380 H383 E384 E406 E413 Y414 L429 P432 G433 D434 Y436 R437 A440 Y472 L564 Y660 N664 Q693 S698	A284 G285 A286 E313 V318 H321 E322 E344 E351 Y352 L367 P370 G371 D372 Y374 R375 A378 Y410 L502 Y598 N602 Q631 S636
BS02	ZN	A	H383 H387 E406	H321 H325 E344

Gene Ontology

Molecular Function

• GO:0001618 virus receptor activity
• GO:0004177 aminopeptidase activity
• GO:0008237 metallopeptidase activity
• GO:0008270 zinc ion binding
• GO:0042277 peptide binding
• GO:0046872 metal ion binding
• GO:0070006 metalloaminopeptidase activity

Biological Process

• GO:0001525 angiogenesis
• GO:0006508 proteolysis
• GO:0030154 cell differentiation
• GO:0043171 peptide catabolic process
• GO:0046718 symbiont entry into host cell

Cellular Component

• GO:0005615 extracellular space
• GO:0005737 cytoplasm
• GO:0005886 plasma membrane

External links

• PDB: RCSB:4hom, PDBe:4hom, PDBj:4hom
• PDBsum: 4hom
• PubMed: 23071329
• UniProt: P15145|AMPN_PIG Aminopeptidase N (Gene Name=ANPEP)