Structure of PDB 4hno Chain A

Receptor sequence

>4hnoA (length=285) Species: 243274 (Thermotoga maritima MSB8)

MIKIGAHMPISKGFDRVPQDTVNIGGNSFQIFPHNARSWSAKLPSDEAAT
KFKREMKKHGIDWENAFCHSGYLINLASPKDDIWQKSVELLKKEVEICRK
LGIRYLNIHPGSHLGTGEEEGIDRIVRGLNEVLNNTEGVVILLENVSQKG
GNIGYKLEQLKKIRDLVDQRDRVAITYDTCHGFDSGYDITKKEGVEALLN
EIESLFGLERLKMIHLNDSKYPLGAAKDRHERIGSGFIGEEGFAVFFSFK
EIQEVPWILETPGGNEEHAEDIKKVFEIIEKFGIE

3D structure

• PDB: 4hno Conserved Structural Chemistry for Incision Activity in Structurally Non-homologous Apurinic/Apyrimidinic Endonuclease APE1 and Endonuclease IV DNA Repair Enzymes.
• Chain: A
• Resolution: 0.9194 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): R37 H69 Y72 H109 E144 D178 H181 H215 D228 H230 E260
• Catalytic site (residue number reindexed from 1): R37 H69 Y72 H109 E144 D178 H181 H215 D228 H230 E260
• Enzyme Commision number: 3.1.21.2: deoxyribonuclease IV.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	A	H69 H109 E144	H69 H109 E144
BS02	MN	A	H181 D228 H230	H181 D228 H230
BS03	ZN	A	H181 D228 H230	H181 D228 H230
BS04	MN	A	E144 D178 H215 E260	E144 D178 H215 E260
BS05	ZN	A	E144 D178 H215 E260	E144 D178 H215 E260

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003906 DNA-(apurinic or apyrimidinic site) endonuclease activity
• GO:0004519 endonuclease activity
• GO:0008081 phosphoric diester hydrolase activity
• GO:0008270 zinc ion binding
• GO:0008833 deoxyribonuclease IV (phage-T4-induced) activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006281 DNA repair
• GO:0006284 base-excision repair

External links

• PDB: RCSB:4hno, PDBe:4hno, PDBj:4hno
• PDBsum: 4hno
• PubMed: 23355472
• UniProt: Q9WYJ7|END4_THEMA Probable endonuclease 4 (Gene Name=nfo)