Structure of PDB 4h8u Chain A

Receptor sequence

>4h8uA (length=554) Species: 1071045 (Rhizobium sp. MX-45)

APWWKSAVFYQVYPRSFKDTNGDGIGDFKGLTEKLDYLKGLGIDAIWINP
HYASPNTDNGYDISDYREVMKEYGTMEDFDRLMAELKKRGMRLMVDVVIN
HSSDQHEWFKSSRASKDNPYRDYYFWRDGKDGHEPNNYPSFFGGSAWEKD
PVTGQYYLHYFGRQQPDLNWDTPKLREELYAMLRFWLDKGVSGMRFATVA
TYSKTPGFPDLTPEQMKNFAEAYTQGPNLHRYLQEMHEKVFDHYDAVTAG
EIFGAPLNQVPLFIDSRRKELDMAFTFDLIRYDRALDRWHTIPRTLADFR
QTIDKVDAIAGEYGWNTFFLGNHDNPRAVSHFGDDRPQWREASAKALATV
TLTQRGTPFIFQGDELGMTNYPFKTLQDFDDIEVKGFFQDYVETGKATAE
ELLTNVALTSRNNARTPFQWDDSANAGFTTGKPWLKVNPNYTEINAAREI
GDPKSVYSFYRNLISIRHETPALSTGSYRDIDPSNADVYAYTRSQDGETY
LVVVNFKAEPRSFTLPDGMHIAETLIESSSPAAPAAGAASLELQPWQSGI
YKVK

3D structure

• PDB: 4h8u Insights into product binding in sucrose isomerases from crystal structures of MutB from Rhizobium sp.
• Chain: A
• Resolution: 2.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D99 R198 A200 E254 H326 D327
• Catalytic site (residue number reindexed from 1): D96 R195 A197 E251 H323 D324
• Enzyme Commision number: 5.4.11.99

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	A	D61 Y64 H104 F164 H326 D327 R414	D58 Y61 H101 F161 H323 D324 R411
BS02	FRU	A	F164 E254 F256 F280 R284 D327 E386	F161 E251 F253 F277 R281 D324 E383
BS03	CA	A	D22 N24 D26 I28 D30	D19 N21 D23 I25 D27

Gene Ontology

Molecular Function

• GO:0004556 alpha-amylase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0009313 oligosaccharide catabolic process

External links

• PDB: RCSB:4h8u, PDBe:4h8u, PDBj:4h8u
• PDBsum: 4h8u
• UniProt: M1E1F6