Structure of PDB 4h8a Chain A

Receptor sequence

>4h8aA (length=337) Species: 562 (Escherichia coli)

MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSHGAVRVE
YYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKT
AQQNGVAVVGISRMGHSGAISYFVQQAARAGFIGISMCQSDPMVVPFGGA
EIYYGTNPLAFAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWA
VDKNGVPTTDPFAVHALLPAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSS
MYDDLHAGRNLGQLHIVINPNFFSSSELFRQHLSQTMRELNAITPAPGFN
QVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISDALY

3D structure

• PDB: 4h8a Structural and functional insights into (s)-ureidoglycolate dehydrogenase, a metabolic branch point enzyme in nitrogen utilization.
• Chain: A
• Resolution: 1.64 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H44
• Catalytic site (residue number reindexed from 1): H44
• Enzyme Commision number: 1.1.1.350: ureidoglycolate dehydrogenase (NAD(+)).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAI	A	I41 H44 H116 G118 I120 S140 T156 P158 F173 D174 A176 Y303 G306 D308 Q309	I41 H44 H116 G118 I120 S140 T156 P158 F173 D174 A176 Y303 G306 D308 Q309
BS02	NAI	A	F147 P223 K224	F147 P223 K224

Gene Ontology

Molecular Function

• GO:0009040 ureidoglycolate dehydrogenase activity
• GO:0016491 oxidoreductase activity

Biological Process

• GO:0000256 allantoin catabolic process
• GO:0006144 purine nucleobase metabolic process
• GO:0009442 allantoin assimilation pathway

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:4h8a, PDBe:4h8a, PDBj:4h8a
• PDBsum: 4h8a
• PubMed: 23284870
• UniProt: P77555|ALLD_ECOLI Ureidoglycolate dehydrogenase (NAD(+)) (Gene Name=allD)