Structure of PDB 4h4u Chain A

Receptor sequence

>4h4uA (length=401) Species: 358220 (Acidovorax sp. KKS102)

ALKAPVVVLGAGLASVSFVAELRQAGYQGLITVVGDEAERPYDRPPLSKD
FMAHGDAEKIRLDCKRAPEVEWLLGVTAQSFDPQAHTVALSDGRTLPYGT
LVLATGAAPRALPTLQGATMPVHTLRTLEDARRIQAGLRPQSRLLIVGGG
VIGLELAATARTAGVHVSLVERQPRLMSRAAPATLADFVARYHAAQGVDL
RFERSVTGSVDGVVLLDDGTRIAADMVVVGIGVLANDALARAAGLACDDG
IFVDAYGRTTCPDVYALGDVTRQRNPLSGRFERIETWSNAQNQGIAVARH
LVDPTAPGYAELPWYWSDQGALRIQVAGLASGDEEIVRGEVSLDAPKFTL
IELQKGRIVGATCVNNARDFAPLRRLLAVGAKPDRAALADPATDLRKLAA
A

3D structure

• PDB: 4h4u Random Mutagenesis with the Project Assessment for Complete Conversion of Co-Factor Specificity of a Ferredoxin Reductase BphA4
• Chain: A
• Resolution: 1.6 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): L17 R48 P49 Q295
• Catalytic site (residue number reindexed from 1): L13 R44 P45 Q291
• Enzyme Commision number: 1.18.1.3: ferredoxin--NAD(+) reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NDP	A	V155 I156 E175 R176 Q177 G234 I235 G236 I288 E289 Y319	V151 I152 E171 R172 Q173 G230 I231 G232 I284 E285 Y315
BS02	FAD	A	G16 A18 D40 R48 P49 A82 T109 G110 L129 R130 I156 E159 G272 D273 E289 T290 W291 A294 W320	G12 A14 D36 R44 P45 A78 T105 G106 L125 R126 I152 E155 G268 D269 E285 T286 W287 A290 W316

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0016491 oxidoreductase activity
• GO:0016651 oxidoreductase activity, acting on NAD(P)H
• GO:0051213 dioxygenase activity

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:4h4u, PDBe:4h4u, PDBj:4h4u
• PDBsum: 4h4u
• UniProt: E7FJB9