Structure of PDB 4h2y Chain A

Receptor sequence

>4h2yA (length=295) Species: 224911 (Bradyrhizobium diazoefficiens USDA 110)

DPLDHLADKLFHSMGSDGVYARTALYESIVERLAALITSHREAGTEALRF
PPVMSRAQLEKSGYLKSFPNLLGCVCGLHGTEREINAAVSRFDAGGDWTT
SLSPADLVLSPAACYPVYPIAASRGPLPKGGLRFDVAADCFRREPSKHLD
RLQSFRMREYVCIGTPDDVSDFRERWMVRAQAIARDLGLTFRVDYASDPF
FGRAGKMLANNQRDQQLKFELLIPLRSEEQPTACMSFNYHREHFGTTWGI
QDANGEPAHTGCVAFGMDRLAVAMFHTHGTDLSAWPAKVRDILGL

3D structure

• PDB: 4h2y Adaptation of aminoacyl-tRNA synthetase catalytic core to carrier protein aminoacylation.
• Chain: A
• Resolution: 2.1 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C131 R159 E161 R168 E176 K235 A250 S253 C279 R286
• Catalytic site (residue number reindexed from 1): C114 R142 E144 R151 E159 K218 A233 S236 C262 R269
• Enzyme Commision number: 6.2.1.n2: amino acid--[acyl-carrier-protein] ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	A	C131 E176 C279	C114 E159 C262
BS02	ATP	A	R159 R168 L169 F172 K235 A250 C251 G283 R286	R142 R151 L152 F155 K218 A233 C234 G266 R269
BS03	PNS	A	D215 N228 Q232 N255 H257	D198 N211 Q215 N238 H240

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004812 aminoacyl-tRNA ligase activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016874 ligase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006418 tRNA aminoacylation for protein translation

External links

• PDB: RCSB:4h2y, PDBe:4h2y, PDBj:4h2y
• PDBsum: 4h2y
• PubMed: 23541895
• UniProt: Q89VT8|AACL1_BRADU Amino acid--[acyl-carrier-protein] ligase 1 (Gene Name=bll0957)